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Endothelin-1 Antibody (TR.ET.48.5) [Janelia Fluor® 585]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ca, Rb, ShSpecies Glossary
Applications WB, Flow, Func, ICC/IF, IHC
Clone
TR.ET.48.5
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 585

Order Details

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Endothelin-1 Antibody (TR.ET.48.5) [Janelia Fluor® 585] Summary

Immunogen
Pure endothelin-1 conjugated to KLH.
Epitope
Studies suggest that this antibody binds to an epitope in the region of ET-1 represented by amino acids 8-16.
Localization
Secreted
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EDN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Radioimmunoassay
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 11673223). Rabbit reactivity reported in scientific literature (PMID: 12181591). Porcine reactivity reported in scientific literature (PMID: 21587116). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Endothelin-1 Antibody (TR.ET.48.5) [Janelia Fluor® 585]

  • ARCND3
  • EDN1
  • endothelin 1
  • Endothelin-1
  • ET1
  • HDLCQ7
  • PPET1
  • preproendothelin-1
  • QME

Background

Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms - ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Endothelin-1 Antibody (NB300-526JF585) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EDN1