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ECE-1 Antibody

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MCF‑7 human breast cancer cell line was stained with Human ECE‑1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF1784, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
MCF‑7 human breast cancer cell line was stained with Human ECE‑1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF1784, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), ...read more
ECE‑1 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human ECE‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1784) at 15 µg/mL overnight at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP, CyTOF-ready, Flow
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF1784
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Catalog# & Formulation Size Price
Carrier Free
AF1784-SP
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ECE-1 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ECE-1
Gln90-Trp770
Accession # P42892
Specificity
Detects human ECE-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human ECE-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ECE1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF1784 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ECE-1 Antibody

  • EC 3.4.24
  • EC 3.4.24.71
  • ECE
  • ECE1
  • ECE-1
  • endothelin converting enzyme 1
  • endothelin-converting enzyme 1

Background

Endothelin-converting Enzyme 1 (ECE-1) is a zinc protease of the neprilysin (NEP) family, which also includes ECE-2, PEX, XCE, DINE, Kell and several NEP-like proteins (1). ECE-1 is a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Four alternatively spliced isoforms differ in their cytoplasmic tail (2, 3). In addition to big endothelin-1, ECE-1 cleaves a variety of bioactive peptides such as bradykinin, neurotensin, angiotensin I, and substance P (1). Together with ECE-2, it is also involved in degradation of beta -amyloid peptide (4). The ectodomain of human ECE-1, which is common to all isoforms, was expressed with an N-terminal His tag and purified.

  1. Turner, A.J. et al. (2001) BioEssays 23:261.
  2. Valdennaire, O. et al. (1999) Eur. J. Biochem. 264:341.
  3. Schweizer, A. et al. (1997) Biochem. J. 328:871.
  4. Eckman, E.A. et al. (2003) J. Biol. Chem. 278:2081.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ECE-1 Antibody (AF1784)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC, IHC, Western Blot.


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ICC
(3)
IHC
(3)
Western Blot
(4)
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(4)
Mouse
(2)
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Showing Publications 1 - 8 of 8.
Publications using AF1784 Applications Species
Q Yu, K Li, A Zhao, M Wei, Z Huang, Y Zhang, Y Chen, L Ting, W Chuan, L Xu, Y Zhang, C Xu, F Liu Sorafenib not only impairs endothelium-dependent relaxation but also promotes vasoconstriction through the upregulation of vasoconstrictive endothelin type B receptors Toxicology and Applied Pharmacology, 2021-01-24;414(0):115420. 2021-01-24 [PMID: 33503445] (Western Blot, Rat) Western Blot Rat
W Chiha, CJ LeVaillant, CA Bartlett, AW Hewitt, PE Melton, M Fitzgerald, AR Harvey Retinal genes are differentially expressed in areas of primary versus secondary degeneration following partial optic nerve injury PLoS ONE, 2018-02-09;13(2):e0192348. 2018-02-09 [PMID: 29425209] (IHC, Rat) IHC Rat
JS Miners, S Love Endothelin-converting enzymes degrade ?-synuclein and are reduced in dementia with Lewy bodies J. Neurochem, 2017-03-29;0(0):. 2017-03-29 [PMID: 28171705] (ICC, Human) ICC Human
Whyteside A, Turner A, Lambert D Endothelin-converting enzyme-1 (ECE-1) is post-transcriptionally regulated by alternative polyadenylation. PLoS ONE, 2014-01-31;9(1):e83260. 2014-01-31 [PMID: 24497914] (Western Blot, Human) Western Blot Human
Zhao P, Canals M, Murphy J, Klingler D, Eriksson E, Pelayo J, Hardt M, Bunnett N, Poole D Agonist-biased trafficking of somatostatin receptor 2A in enteric neurons. J Biol Chem, 2013-08-02;288(36):25689-700. 2013-08-02 [PMID: 23913690] (IHC, Mouse, Rat) IHC Mouse, Rat
Wang S, Wang R, Chen L, Bennett DA, Dickson DW, Wang DS Expression and functional profiling of neprilysin, insulin-degrading enzyme, and endothelin-converting enzyme in prospectively studied elderly and Alzheimer's brain. J. Neurochem., 2010-07-30;115(1):47-57. 2010-07-30 [PMID: 20663017] (Western Blot, Human) Western Blot Human
Cottrell GS, Padilla BE, Amadesi S, Poole DP, Murphy JE, Hardt M, Roosterman D, Steinhoff M, Bunnett NW Endosomal endothelin-converting enzyme-1: a regulator of beta-arrestin-dependent ERK signaling. J. Biol. Chem., 2009-06-16;284(33):22411-25. 2009-06-16 [PMID: 19531493] (ICC, IHC, Mouse, Rat) ICC, IHC Mouse, Rat
Padilla BE, Cottrell GS, Roosterman D, Pikios S, Muller L, Steinhoff M, Bunnett NW Endothelin-converting enzyme-1 regulates endosomal sorting of calcitonin receptor-like receptor and beta-arrestins. J. Cell Biol., 2007-11-26;179(5):981-97. 2007-11-26 [PMID: 18039931] (ICC, Western Blot, Human) ICC, Western Blot Human

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Bioinformatics

Gene Symbol ECE1
Uniprot