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Endoglycan/PODXL2 Antibody

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Endoglycan/PODXL2 was detected in perfusion fixed frozen sections of mouse testis using 1.7 µg/mL Goat Anti-Mouse Endoglycan/PODXL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3534) overnight at 4 °C. ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Endoglycan/PODXL2 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Endoglycan/PODXL2
Gly30-Gly498
Accession # Q8CAE9
Specificity
Detects mouse Endoglycan/PODXL2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PODXL2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF3534 in the following applications:

  • IHC
    3 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Endoglycan/PODXL2 Antibody

  • Endoglycan
  • PODLX2
  • podocalyxin-like 2
  • podocalyxin-like protein 2
  • PODXL2

Background

Endoglycan, also named podocalyxin-like 2 protein, is a type I transmembrane glycoprotein that belongs to the CD34/podocalyxin family of sialomucins that are expressed on endothelial cells and hematopoietic precursors, vascular smooth muscle cells, and some leukocyte subpopulations. The extracellular domain of Endoglycan contains an N-terminal acidic domain with several polyglutamate tracts, a mucin-like domain and a membrane proximal globular domain. Unlike other sialomucins, Endoglycan contains chondroitin sulfate modifications. Within the extracellular domain, mouse endoglycan shares 73% and 96% amino acid sequence identity with human and rat endoglycan, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Endoglycan/PODXL2 Antibody (AF3534)(3)

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Bioinformatics

Gene Symbol PODXL2
Uniprot