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Recombinant Human EMAP-II/AIMP1 Protein

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SDS-Page: Recombinant Human EMAP-II/AIMP1 Protein [NBP2-34954] - SDS-PAGE of NBP2-34954, showing a band at approximately 18 kDa.
SDS-Page: Recombinant Human EMAP-II/AIMP1 Protein [NBP2-34954] - SDS-PAGE of NBP2-34954, showing a band at approximately 18 kDa.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier-Free
NBP2-34954-1mg
Carrier-Free
NBP2-34954-100ug
Carrier-Free
NBP2-34954-500ug
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Recombinant Human EMAP-II/AIMP1 Protein Summary

Description
A single non-glycosylated polypeptide chain containing 166 amino acids corresponding to EMAP-II/AIMP1 Source: E. coli

Uniprot ID: Q12904

Amino Acid Sequence: SKPIDVSRLD LRIGCIITAR KHPDADSLYV EEVDVGEIAP RTVVSGLVNH VPLEQMQNRM VILLCNLKPA KMRGVLSQAM VMCASSPEKI EILAPPNGSV PGDRITFDAF PGEPDKELNP KKKIWEQIQP DLHTNDECVA TYKGVPFEVK GKGVCRAQTM SNSGIK
Details of Functionality
Fully biologically active when compared to standard. The ED50 as determined by the apoptotic effect using serum free human MCF-7 cells is less than 40 ng/ml, corresponding to a specific activity of > 2.5 x 10^4 IU/mg.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
AIMP1
Purity
> 98 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of EMAP-II/AIMP1 as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
18.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative
No Preservative
Concentration
LYOPH
Purity
> 98 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL

Alternate Names for Recombinant Human EMAP-II/AIMP1 Protein

  • AIMP1
  • aminoacyl tRNA synthetase complex-interacting multifunctional protein 1
  • EMAP-2
  • EMAPII
  • EMAP-II
  • EMAPIImember 1 (endothelialmonocyte-activating)
  • endothelial-monocyte activating polypeptide II
  • Multisynthase complex auxiliary component p43
  • multisynthetase complex auxiliary component p43
  • SCYE1

Background

EMAP-II is a tumor derived cytokine that exerts a wide range of activities on endothelial cells, monocytes and neutrophils. EMAP-II inhibits endothelial cell proliferation, vasculogenesis, neovessel formation, and can induce apoptosis. It is also chemotactic towards neutrophils and monocytes and induces myeloperoxidase activity from neutrophils. Of clinical importance, EML2 (EMAP-II) inhibits angiogenesis of vascular beds and suppresses the growth of primary and secondary tumors without affecting normal tissues. Mature EMAP-II is an 18.3 kDa protein, which is synthesized as the C-terminal portion of a biologically inactive precursor protein containing a propeptide of 146 amino acid residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol AIMP1
Uniprot