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Elongin A Antibody

Images

 
Western Blot: Elongin A Antibody [NBP1-87040] - Analysis in human cell line U-2197.
Immunocytochemistry/ Immunofluorescence: Elongin A Antibody [NBP1-87040] - Staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Elongin A Antibody [NBP1-87040] - Staining of human liver shows moderate to strong nuclear positivity in hepatocytes.
Immunohistochemistry-Paraffin: Elongin A Antibody [NBP1-87040] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Elongin A Antibody [NBP1-87040] - Staining of human placenta shows moderate nuclear positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Elongin A Antibody [NBP1-87040] - Staining of human rectum shows moderate nuclear positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Elongin A Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ERHLGEPHGKGVVSQNKEHKSSHKDKRPVDAKSDEKASVVSREKSHKALSKEENRRPPSGDNAREKPPSSGVKKEKDREGSSLKKKCLPPSEAASDNHLKKPKHRDPEKAKLDKSKQGLDSFDTGKGAGD
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TCEB3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Elongin A Protein (NBP1-87040PEP)
Publications
Read Publication using
NBP1-87040 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Elongin A Antibody

  • EloA
  • Elongin 110 kDa subunit
  • elongin-A
  • FLJ38760
  • FLJ42849
  • RNA polymerase II transcription factor SIII subunit A1
  • SIII p110
  • SIII
  • TCEB3A
  • transcription elongation factor B (SIII), polypeptide 3 (110kD, elongin A)
  • transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A)
  • transcription elongation factor B alpha subunit
  • transcription elongation factor B polypeptide 3

Background

TCEB3 (Elongin A) is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Elongin A Antibody (NBP1-87040)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IF/IHC, WB.


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(1)
WB
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TCEB3