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ELF3/ESE-1 Antibody

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Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Analysis in human colon and skeletal muscle tissues. Corresponding ELF3 RNA-seq data are presented for the same tissues.
Orthogonal Strategies: Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Analysis in human colon and skeletal muscle tissues. Corresponding ELF3 RNA-seq data are presented for the same tissues.
Independent Antibodies: Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Staining of human colon, endometrium, pancreas and skeletal muscle using Anti-ELF3/ESE-1 antibody NBP1-87945 (A) shows ...read more
Orthogonal Strategies: Western Blot: ELF3/ESE-1 Antibody [NBP1-87945] - Analysis in human cell lines A-431 and SK-MEL-30 using anti-ELF3 antibody. Corresponding ELF3 RNA-seq data are presented for the same cell ...read more
Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Staining of human pancreas shows moderate nuclear positivity in pancreatic ducts.
Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Staining of human colon shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Staining of human endometrium shows moderate to strong nuclear positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

      

Orthogonal Strategies

  

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ELF3/ESE-1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ATCEISNIFSNYFSAMYSSEDSTLASVPPAATFGADDLVLTLSNPQMSLEGTEKASWLGEQPQFWSKTQVLDWISYQVEKNKYDASAIDFSRCDMDGATLCNCALEELRLVFGPLGDQLHAQLRDLTSSSSDELSWIIELLEKDGMA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ELF3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
ELF3/ESE-1 Protein (NBP1-87945PEP)

Reactivity Notes

Mouse (86%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ELF3/ESE-1 Antibody

  • E74-like factor 3 (ets domain transcription factor, epithelial-specific )
  • E74-like factor 3
  • ELF3
  • Epithelial-restricted with serine box
  • epithelial-specific)
  • Epithelium-restricted Ets protein ESX
  • Epithelium-specific Ets transcription factor 1
  • EPR1
  • EPR-1
  • ERT
  • ESX
  • ets domain transcription factor, serine box (epithelial-specific)
  • ETS-related transcription factor Elf-3
  • JEN
  • serine box

Background

ESE-1 (Epithelium-Specific Ets) is a novel, highly tissue-restricted member of the ets transcription factor/oncogene family, which has features distinct from those of any other ets-related factor. ESE-1 binds with high affinity to and transactivates the ets binding site in the promoter of the keratinocyte terminal differentiation marker gene, SPRR2A. ESE-1 is a critical regulator of epithelial cell differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ELF3/ESE-1 Antibody
NBP1-87945
ELF3/ESE-1 Antibody
Species: Hu
Applications: WB, IHC

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FAQs for ELF3/ESE-1 Antibody (NBP1-87945). (Showing 1 - 1 of 1 FAQ).

  1. Do you have any data to indicate that it works for ChIP, and if not are you willing to provide 8 ug for a trial ChIP - we will give shipping charges.
    • We have not tested this antibody for its use in ChIP. While we can not provide you a trial sample, if you would like to test this antibody in an untested application and share your results with us, then I can recommend our Innovators Reward Program.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol ELF3
Entrez
Uniprot