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eIF2B3 Antibody (OTI1A4)

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Western Blot: eIF2B3 Antibody (1A4) [NBP2-01370] Analysis of extracts (35ug) from 9 different cell lines by using anti-eIF2B3 monoclonal antibody.
Western Blot: eIF2B3 Antibody (1A4) [NBP2-01370] Analysis of extracts (35ug) from 9 different cell lines by using anti-eIF2B3 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: eIF2B3 Antibody (1A4) [NBP2-01370] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY eIF2B3.
Immunohistochemistry-Paraffin: eIF2B3 Antibody (1A4) [NBP2-01370] - Staining of paraffin-embedded Human prostate tissue using anti-eIF2B3 mouse monoclonal antibody.
Western Blot: eIF2B3 Antibody (1A4) [NBP2-01370] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY eIF2B3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: eIF2B3 Antibody (1A4) [NBP2-01370] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-eIF2B3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: eIF2B3 Antibody (1A4) [NBP2-01370] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-eIF2B3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: eIF2B3 Antibody (1A4) [NBP2-01370] - Staining of paraffin-embedded Human endometrium tissue using anti-eIF2B3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: eIF2B3 Antibody (1A4) [NBP2-01370] - Staining of paraffin-embedded Human pancreas tissue using anti-eIF2B3 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI1A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.7 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71397AF350
Alexa Fluor 405
NBP2-71397AF405
Alexa Fluor 488
NBP2-71397AF488
Alexa Fluor 532
NBP2-71397AF532
Alexa Fluor 594
NBP2-71397AF594
Alexa Fluor 647
NBP2-71397AF647
Alexa Fluor 700
NBP2-71397AF700
Alexa Fluor 750
NBP2-71397AF750
Allophycocyanin
NBP2-71397APC
Biotin
NBP2-71397B
CoraFluor 1
NBP2-71397CL1
DyLight 350
NBP2-71397UV
DyLight 405
NBP2-71397V
DyLight 488
NBP2-71397G
DyLight 550
NBP2-71397R
DyLight 594
NBP2-71397DL594
DyLight 650
NBP2-71397C
DyLight 680
NBP2-71397FR
DyLight 755
NBP2-71397IR
FITC
NBP2-71397F
HRP
NBP2-71397H
Janelia Fluor 549
NBP2-71397JF549
Janelia Fluor 646
NBP2-71397JF646
PE
NBP2-71397PE
PerCP
NBP2-71397PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-71397
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eIF2B3 Antibody (OTI1A4) Summary

Immunogen
Full length human recombinant protein of human EIF2B3(NP_065098) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EIF2B3
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
50.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.7 mg/ml
Purity
Immunogen affinity purified

Alternate Names for eIF2B3 Antibody (OTI1A4)

  • eIF-2B GDP-GTP exchange factor subunit gamma
  • EIF-2B
  • EIF2Bgamma
  • eukaryotic translation initiation factor 2B, subunit 3 (gamma, 58kD)
  • eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa
  • translation initiation factor eIF-2B subunit gamma

Background

The protein encoded by this gene is one of the subunits of initiation factor eIF2B, which catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. It has also been found to function as a cofactor of hepatitis C virus internal ribosome entry site-mediated translation. Mutations in this gene have been associated with leukodystrophy with vanishing white matter. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-01370
eIF2B3 Antibody (OTI1A4)
Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF, IHC

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Bioinformatics

Gene Symbol EIF2B3
Entrez