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EIF2B2 Antibody

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Western Blot: EIF2B2 Antibody [NBP1-84877] - Analysis in human cell line MCF-7.
Western Blot: EIF2B2 Antibody [NBP1-84877] - Analysis in human cell line MCF-7.
Immunohistochemistry-Paraffin: EIF2B2 Antibody [NBP1-84877] - Analysis in human thyroid gland and liver tissues using NBP1-84877 antibody. Corresponding EIF2B2 RNA-seq data are presented for the same tissues.
Western Blot: EIF2B2 Antibody [NBP1-84877] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Western Blot: EIF2B2 Antibody [NBP1-84877] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: EIF2B2 Antibody [NBP1-84877] - Staining of human stomach shows strong cytoplasmic positivity in chief cells.
Immunohistochemistry-Paraffin: EIF2B2 Antibody [NBP1-84877] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: EIF2B2 Antibody [NBP1-84877] - Staining of human thyroid gland shows high expression.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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EIF2B2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PFCQGHEMAVNLSKAGIETTVMTDAAIFAVMSRVNKVIIGTKTILANGALRAVTGTHTLALAAKHHSTPLIVCAPMFKLSPQFPNEEDSFHKFVAPEEVLPFTEGDILEKVSVHCPVFDYVPPELITLFISNIGGNAPSYIYRLMSELYH
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF2B2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:100-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
EIF2B2 Protein (NBP1-84877PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for EIF2B2 Antibody

  • eIF-2B GDP-GTP exchange factor subunit beta
  • EIF2B
  • EIF2BB
  • EIF-2Bbeta
  • eukaryotic translation initiation factor 2B, subunit 2 (beta, 39kD)
  • eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa
  • S20I15
  • S20III15
  • translation initiation factor eIF-2B subunit beta

Background

Eukaryotic initiation factor-2B (EIF2B) is a GTP exchange protein essential for protein synthesis. It consists of alpha (EIF2B1; MIM 606686), beta (EIF2B2), gamma (EIF2B3; MIM 606273), delta (EIF2B4; MIM 606687), and epsilon (EIF2B5; MIM 603945) subunits. EIF2B activates its EIF2 (see MIM 603907) substrate by exchanging EIF2-bound GDP for GTP.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol EIF2B2