![Knockdown Validated: EFTUD2 Antibody [NB100-40849] - a The protein and mRNA levels of EFTUD2 expression in HCC cells detected by western blot and qPCR. b Western blot analysis of EFTUD2 expression in Hep3B and Huh7 cells infected with siRNA against EFTUD2 (siEFTUD2). e Western blot analysis of EFTUD2 protein expression in Hep3B and Huh7 cells infected with lentiviruses expressing either scrambled shRNA or shRNA against EFTUD2 (shEFTUD2). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-03040-5) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EFTUD2-Antibody-Knockdown-Validated-NB100-40849-img0006.jpg "Knockdown Validated: EFTUD2 Antibody [NB100-40849] - a The protein and mRNA levels of EFTUD2 expression in HCC cells detected by western blot and qPCR. b Western blot analysis of EFTUD2 expression in Hep3B and Huh7 cells infected with siRNA against EFTUD2 (siEFTUD2). e Western blot analysis of EFTUD2 protein expression in Hep3B and Huh7 cells infected with lentiviruses expressing either scrambled shRNA or shRNA against EFTUD2 (shEFTUD2). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-03040-5) licensed under a CC-BY license.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IHC, IP, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human Elongation Factor Tu GTP Binding Domain Containing 2 (U5 snRNP-Specific Protein, 116 kD ) using the numbering given in entry NP_004238.2 (GeneID 9343). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | EFTUD2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
![Western Blot RFC1 Antibody [Unconjugated]](https://images.novusbio.com/images2/RFC1_AF6457_Western_Blot_9874.jpg)
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry: EFTUD2 Antibody [NB100-40849] - Serial sections of human HCC tissue samples were subjected to IHC staining with antibodies against EFTUD2. Representative IHC images from 50 human HCC tissue samples. Scale bars, 100 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-03040-5) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EFTUD2-Antibody-Immunohistochemistry-NB100-40849-img0007.jpg "Immunohistochemistry: EFTUD2 Antibody [NB100-40849] - Serial sections of human HCC tissue samples were subjected to IHC staining with antibodies against EFTUD2. Representative IHC images from 50 human HCC tissue samples. Scale bars, 100 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-03040-5) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: EFTUD2 Antibody [NB100-40849] - Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-EFTUD2/SNRP116 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/EFTUD2-Antibody-Immunohistochemistry-Paraffin-NB100-40849-img0005.jpg "Immunohistochemistry-Paraffin: EFTUD2 Antibody [NB100-40849] - Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-EFTUD2/SNRP116 used at a dilution of 1:200 (1ug/ml). Detection: DAB")
![Immunoprecipitation: EFTUD2 Antibody [NB100-40849] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Affinity purified rabbit anti- EFTUD2/SNRP116 antibody 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate (B).](http://images.novusbio.com/fullsize/EFTUD2-Antibody-Immunoprecipitation-NB100-40849-img0003.jpg "Immunoprecipitation: EFTUD2 Antibody [NB100-40849] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Affinity purified rabbit anti- EFTUD2/SNRP116 antibody 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate (B).")
![Western Blot Osteoactivin/GPNMB Antibody [Unconjugated]](https://images.novusbio.com/images2/Osteoactivin_AF2550_Western_Blot_19926.jpg)
![Immunohistochemistry Osteoactivin/GPNMB Antibody [Unconjugated]](https://images.novusbio.com/images2/Osteoactivin_AF2550_Immunohistochemistry_15337.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")