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EB2 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: EB2 Antibody [NBP1-84927] - Analysis in human cerebral cortex and testis tissues. Corresponding MAPRE2 RNA-seq data are presented for the same tissues.
Independent Antibodies: Immunohistochemistry-Paraffin: EB2 Antibody [NBP1-84927] - Staining of human cerebral cortex, colon, liver and testis using Anti-MAPRE2 antibody NBP1-84927 (A) shows similar protein ...read more
Western Blot: EB2 Antibody [NBP1-84927] - Analysis in control (vector only transfected HEK293T lysate) and MAPRE2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: EB2 Antibody [NBP1-84927] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.
Immunohistochemistry-Paraffin: EB2 Antibody [NBP1-84927] - Staining of human cerebral cortex shows high expression.
Immunohistochemistry-Paraffin: EB2 Antibody [NBP1-84927] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: EB2 Antibody [NBP1-84927] - Staining of human colon shows moderate cytoplasmic positivity in peripheral nerve / ganglion.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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EB2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SSSGSASKSDKDLETQVIQLNEQVHSLKLALEGVEKERDFYFGKLREIELLCQEHGQENDDLVQ
Predicted Species
Mouse (98%), Rat (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAPRE2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
EB2 Protein (NBP1-84927PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for EB2 Antibody

  • APC-binding protein EB2
  • EB1
  • EB2APC-binding protein EB1
  • End-binding protein 2
  • microtubule-associated protein, RP/EB family, member 2
  • RP1microtubule-associated protein RP/EB family member 2
  • T-cell activation protein, EB1 family

Background

EB2 is encoded by this gene shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family. The function of this protein is unknown; however, its homology suggests involvement in tumorigenesis of colorectal cancers and proliferative control of normal cells. This gene may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAPRE2