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EARS2 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: EARS2 Antibody [NBP1-91856] - Immunofluorescent staining of human cell line Hep G2 shows localization to mitochondria.
Immunohistochemistry-Paraffin: EARS2 Antibody [NBP1-91856] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules and cells in glomeruli.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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EARS2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LLDLEKLPEFNRLHLQRLVSNESQRRQLVGKLQVLVEEAFGCQLQNRDVLNPVYVERILLLRQGHICRLQDLVSPVYSYLWTRPAVGRAQ
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EARS2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
EARS2 Protein (NBP1-91856PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (81%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for EARS2 Antibody

  • EC 6.1.1.17
  • gluRS
  • Glutamate--tRNA ligase
  • glutamyl-tRNA synthetase 2, mitochondrial (putative)
  • KIAA1970glutamate tRNA ligase 2, mitochondrial
  • MSE1
  • probable glutamyl-tRNA synthetase, mitochondrial

Background

EARS2 catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional EARS2 Products

Array NBP1-91856

Research Areas for EARS2 Antibody (NBP1-91856)

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Bioinformatics

Gene Symbol EARS2