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Dysferlin Antibody (CL10888)

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Dysferlin Antibody (CL10888) [NBP3-07994] - Analysis in human spleen and skin tissues using NBP3-07994 antibody. Corresponding Dysferlin RNA-seq data are ...read more
Western Blot: Dysferlin Antibody (CL10888) [NBP3-07994] - Analysis in human cell line RH-30.
Immunohistochemistry-Paraffin: Dysferlin Antibody (CL10888) [NBP3-07994] - Staining of human spleen shows moderate positivity in cells in red pulp.
Immunohistochemistry-Paraffin: Dysferlin Antibody (CL10888) [NBP3-07994] - Staining of human lymph node shows strong membranous positivity in non-germinal center cells and endothelial cells.
Immunohistochemistry-Paraffin: Dysferlin Antibody (CL10888) [NBP3-07994] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Dysferlin Antibody (CL10888) [NBP3-07994] - Staining of human skin shows no positivity in keratinocytes as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
CL10888
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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Dysferlin Antibody (CL10888) Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: CHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALA
Epitope
VDSLVAQLTDELIAG
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DYSF
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Mouse, Rat (88%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, containing 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Dysferlin Antibody (CL10888)

  • dysferlin
  • dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive)
  • dystrophy-associated fer-1-like 1
  • FER1L1Dystrophy-associated fer-1-like protein
  • Fer-1-like protein 1
  • FLJ00175
  • FLJ90168
  • LGMD2B

Background

Dysferlin is the protein product of the 2p13 gene that is defective in patients with Limb-Girdle Muscular Dystrophy type 2B (LGMD2B) and Miyoshi Myopathy (MM). Dysferlin is normally localized to the muscle plasma membrane. In patients with LGMD2B and MM, immunoreactivity to dysferlin is severely reduced or lost, depending on the type of mutation. This antibody is used for the characterization of LGMD2B and MM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DYSF