![Immunohistochemistry-Paraffin: DPF3 Antibody [NBP2-49494] -Analysis in human cerebral cortex and prostate tissues using NBP2-49494 antibody. Corresponding DPF3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp2-49494_rabbit-polyclonal-dpf3-antibody-immunohistochemistry-paraffin-12920241166..jpg "Immunohistochemistry-Paraffin: DPF3 Antibody [NBP2-49494] -Analysis in human cerebral cortex and prostate tissues using NBP2-49494 antibody. Corresponding DPF3 RNA-seq data are presented for the same tissues.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against a recombinant protein corresponding to amino acids: KLRLLEIKPEVELPLKKDGFTSESTTLEALLRGEGVEKKVDAREEESIQEIQRVLENDENVEEGNEEEDLEEDIPKRKNRTRGRA |
| Predicted Species | Mouse (99%), Rat (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DPF3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | DPF3 |
![Immunocytochemistry/Immunofluorescence: DPF3 Antibody [NBP2-49494] - Staining of human cell line BJ shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DPF3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49494-img0002.jpg "Immunocytochemistry/Immunofluorescence: DPF3 Antibody [NBP2-49494] - Staining of human cell line BJ shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: DPF3 Antibody [NBP2-49494] -Analysis in human cerebral cortex and prostate tissues using NBP2-49494 antibody. Corresponding DPF3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp2-49494_rabbit-polyclonal-dpf3-antibody-immunohistochemistry-paraffin-129202411245.jpg "Immunohistochemistry-Paraffin: DPF3 Antibody [NBP2-49494] -Analysis in human cerebral cortex and prostate tissues using NBP2-49494 antibody. Corresponding DPF3 RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: DPF3 Antibody [NBP2-49494] -Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/antibody/nbp2-49494_rabbit-polyclonal-dpf3-antibody-immunohistochemistry-paraffin-129202411738..jpg "Immunohistochemistry-Paraffin: DPF3 Antibody [NBP2-49494] -Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.")
![Immunohistochemistry-Paraffin: DPF3 Antibody [NBP2-49494] -Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/antibody/nbp2-49494_rabbit-polyclonal-dpf3-antibody-immunohistochemistry-paraffin-129202411105..jpg "Immunohistochemistry-Paraffin: DPF3 Antibody [NBP2-49494] -Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.")
![Immunohistochemistry-Paraffin: DPF3 Antibody [NBP2-49494] -Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-49494_rabbit-polyclonal-dpf3-antibody-immunohistochemistry-paraffin-1292024111131..jpg "Immunohistochemistry-Paraffin: DPF3 Antibody [NBP2-49494] -Staining of human prostate shows no positivity in glandular cells as expected.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")