![Western Blot: PHF10 Antibody [NBP2-19795] - HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PHF10 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PHF10-Antibody-Western-Blot-NBP2-19795-img0005.jpg "Western Blot: PHF10 Antibody [NBP2-19795] - HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PHF10 antibody diluted at 1:1000.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PHF10. The exact sequence is proprietary. |
| Localization | Nucleus |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PHF10 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 56 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Reviewed Applications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
-(01-ml)_NBP2-19795_9176.jpg)
Enlarge |
reviewed by:
Xiaofeng Wang |
WB | Human | 08/07/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: PHF10 Antibody [NBP2-19795] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PHF10 protein stained by PHF10 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/PHF10-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19795-img0004.jpg "Immunocytochemistry/Immunofluorescence: PHF10 Antibody [NBP2-19795] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PHF10 protein stained by PHF10 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: PHF10 Antibody [NBP2-19795] - Mouse lung. PHF10 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PHF10-Antibody-Immunohistochemistry-Paraffin-NBP2-19795-img0007.jpg "Immunohistochemistry-Paraffin: PHF10 Antibody [NBP2-19795] - Mouse lung. PHF10 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: PHF10 Antibody [NBP2-19795] - 50 ug mouse colon lysate/extract 10% SDS-PAGE PHF10 antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/PHF10-Antibody-Western-Blot-NBP2-19795-img0001.jpg "Western Blot: PHF10 Antibody [NBP2-19795] - 50 ug mouse colon lysate/extract 10% SDS-PAGE PHF10 antibody dilution: 1:1000.")
![Immunohistochemistry-Paraffin: PHF10 Antibody [NBP2-19795] - Hela xenograft, using PHF10 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PHF10-Antibody-Immunohistochemistry-Paraffin-NBP2-19795-img0003.jpg "Immunohistochemistry-Paraffin: PHF10 Antibody [NBP2-19795] - Hela xenograft, using PHF10 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: PHF10 Antibody [NBP2-19795] - Rat lung. PHF10 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PHF10-Antibody-Immunohistochemistry-Paraffin-NBP2-19795-img0006.jpg "Immunohistochemistry-Paraffin: PHF10 Antibody [NBP2-19795] - Rat lung. PHF10 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry DLL1 Antibody [Unconjugated]](https://images.novusbio.com/images2/DLL1_AF3970_Immunohistochemistry_6817.jpg)
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![Immunocytochemistry/ Immunofluorescence DLL1 Antibody [Unconjugated]](https://images.novusbio.com/images/af3970_mouse-rat-dll1-affinity-purified-polyclonal-ab-41202412403596.jpg)
![Western Blot Brg1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Brg1_AF5738_Western_Blot_5829.jpg)
![Immunocytochemistry Brg1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Brg1_AF5738_Immunocytochemistry_11663.jpg)
![Immunocytochemistry Brg1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Brg1_AF5738_Immunocytochemistry_11664.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
-(01-ml)_NBP2-19795_9176.jpg)