Novus Biologicals products are now on bio-techne.com

Dopa Decarboxylase/DDC Antibody

Images

 
Western Blot: Dopa Decarboxylase/DDC Antibody [NBP1-56918] - Reccomended Titration: 1 ug/ml Positive Control: Human 293T cell lysate
Immunohistochemistry-Paraffin: Dopa Decarboxylase/DDC Antibody [NBP1-56918] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X ...read more
Western Blot: Dopa Decarboxylase/DDC Antibody [NBP1-56918] - HepG2 cell lysate, Antibody Titration: 5.0ug/ml

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Dopa Decarboxylase/DDC Antibody Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptides corresponding to DDC (dopa decarboxylase, aromatic L-amino acid decarboxylase). The peptide sequence was selected from the N terminal of DDC (NP_000781). Peptide sequence EFRRRGKEMVDYVANYMEGIEGRQVYPDVEPGYLRPLIPAAAPQEPDTFE. The peptide sequence for this immunogen was taken from within the described region.
Localization
Cytosol
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDC
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 4-8 ug/ml
  • Western Blot 1.0 ug/ml
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Dopa Decarboxylase/DDC Protein (NBP1-56918PEP)
Publications
Read Publications using
NBP1-56918 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29789702).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Dopa Decarboxylase/DDC Antibody

  • AADC
  • AADCaromatic-L-amino-acid decarboxylase
  • DDC
  • dopa decarboxylase (aromatic L-amino acid decarboxylase)
  • Dopa Decarboxylase
  • EC 4.1.1
  • EC 4.1.1.28

Background

DDC catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-109
Species: Ch, Dr, Hu, I, Ma, Mu, Rt
Applications: Dual ISH-IHC, ICC/IF, IHC-FrFl, IHC-WhMt, IHC, IHC-Fr, IHC-P, KO, Simple Western, WB
NBP1-31386
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NB100-1780
Species: Hu, Mu
Applications: Flow, PEP-ELISA, WB
NBP2-24915
Species: Bv, Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP2-00688
Species: Ca, Hu, Pm, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AF3398
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
NBP2-22164
Species: Hu(-), Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
AF5724
Species: Hu, Mu, Rt
Applications: ICC, WB
NBP2-33413
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-93574
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-87781
Species: Hu, Mu
Applications: IHC, IHC-P, WB
MAB6734
Species: Hu
Applications: IHC
NBP1-84949
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-59580
Species: Hu, Rt
Applications: WB
NBP1-47977
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-56918
Species: Hu, Mu
Applications: WB, IHC

Publications for Dopa Decarboxylase/DDC Antibody (NBP1-56918)(5)

Reviews for Dopa Decarboxylase/DDC Antibody (NBP1-56918) (0)

There are no reviews for Dopa Decarboxylase/DDC Antibody (NBP1-56918). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Dopa Decarboxylase/DDC Antibody (NBP1-56918). (Showing 1 - 1 of 1 FAQs).

  1. I would like to use your anti-DOPA Decarboxylase Antibody (NBP1-56918) in fixed mouse brain sections (IHC) and was wondering if it has been evaluated for this particular use? Do you also offer the DOPA Decarboxylase Peptide?
    • In our validation studies, NBP1-56918 was tested with human kidney tissue in IHC-P application wherein this antibody showed nice staining at the antibody concentration range of 4-8ug/ml. Unfortunately, I do not have any data to provide on the mouse brain's IHC-P staining specifically at the moment, however, you should not be concerned because the immunogen sequence is 100% identical to mouse and we have validated this product successfully in IHC-P. We are always there to help in case you face any trouble in generating your desired outcome out of the use of our products. We do sell Blocking Peptide specific for DOPA Decarboxylase Antibody (NBP1-56918) and the catalog # for the peptide is NBP1-56918PEP.

Secondary Antibodies

 

Isotype Controls

Additional Dopa Decarboxylase/DDC Products

Research Areas for Dopa Decarboxylase/DDC Antibody (NBP1-56918)

Find related products by research area.

Blogs on Dopa Decarboxylase/DDC

There are no specific blogs for Dopa Decarboxylase/DDC, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Dopa Decarboxylase/DDC Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol DDC
Entrez
Uniprot