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Dopa Decarboxylase/DDC Antibody (CL2962)

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Dopa Decarboxylase/DDC Antibody (CL2962) [NBP2-46642] - Staining in human kidney and fallopian tube tissues. Corresponding DDC RNA-seq data are presented for ...read more
Western Blot: Dopa Decarboxylase/DDC Antibody (CL2962) [NBP2-46642] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Kidney tissue
Immunohistochemistry: Dopa Decarboxylase/DDC Antibody (CL2962) [NBP2-46642] - Staining of mouse substantia nigra shows strong positivity in dopamine neurons in pars compacta.
Immunohistochemistry-Paraffin: Dopa Decarboxylase/DDC Antibody (CL2962) [NBP2-46642] - Staining of human substantia nigra shows cytoplasmic positivity in dopamine neurons in pars compacta.
Immunohistochemistry-Paraffin: Dopa Decarboxylase/DDC Antibody (CL2962) [NBP2-46642] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: Dopa Decarboxylase/DDC Antibody (CL2962) [NBP2-46642] - Staining of human fallopian tube shows no positivity in glandular cells as expected.
Immunohistochemistry: Dopa Decarboxylase/DDC Antibody (CL2962) [NBP2-46642] - Staining of rat substantia nigra shows strong positivity in dopamine neurons in pars compacta.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
CL2962
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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Dopa Decarboxylase/DDC Antibody (CL2962) Summary

Immunogen
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence LETVMMDWLGK mlELPKAFLNEKAGEGGGVIQGSASEATLVALLAARTKVIHRLQAASPELTQAAIMEKLVAYSSDQAHSSVERAGLIGGVKLKAIPSDGNFAMRASA
Epitope
MDWLGKMLEL
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DDC
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Dopa Decarboxylase/DDC Recombinant Protein Antigen (NBP2-46642PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Dopa Decarboxylase/DDC Antibody (CL2962)

  • AADC
  • AADCaromatic-L-amino-acid decarboxylase
  • DDC
  • dopa decarboxylase (aromatic L-amino acid decarboxylase)
  • Dopa Decarboxylase
  • EC 4.1.1
  • EC 4.1.1.28

Background

DOPA Decarboxylase (aromatic L-amino acid decarboxylase, AADC; DDC) catalyzes the second reaction in the biosynthesis of catecholamines and serotonin (Waymire and Haycock, 2002; Berry et al., 1996; Haycock et al., 2003). It is also involved in the biosynthesis of trace amines. DDC antibodies can therefore be used as markers for dopaminergic, noradrenergic and serotonergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DDC
Entrez
Uniprot