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DLST Antibody

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Biological Strategies: Western Blot: DLST Antibody [NBP2-32204] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit ...read more
Immunohistochemistry-Paraffin: DLST Antibody [NBP2-32204] - section of human lung cancer. Antibody: Affinity purified rabbit anti- DLSTused at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: DLST Antibody [NBP2-32204] - section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- DLST used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: DLST Antibody [NBP2-32204] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-DLST antibody NBP2-32204 used ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

DLST Antibody Summary

Immunogen
The immunogen this antibody was made to, maps to a region between residue 50 to 100 of human Dihydrolipoamide S-succinyltransferase using the numbering given in entry NP_001924.2 (GeneID 1743).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DLST
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DLST Antibody

  • 2-oxoglutarate dehydrogenase complex component E2
  • dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutaratecomplex)
  • Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenasecomplex
  • dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutaratedehydrogenase complex, mitochondrial
  • DLTS
  • E2K
  • EC 2.3.1
  • EC 2.3.1.61
  • OGDC-E2

Background

DLST, also known as Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, is a 48.8 kDa, 453 amino acid protein that is involved in catalyzing the reaction of 2-oxoglutarate into succinyl-CoA and carbon dioxide. Current research is investigating the effect of the protein on malaria, neurodegeneration, pneumonia, cat-scratch disease, ataxia, prostatitis, Parkinson's disease, and Alzheimer's disease. The protein is involved in lysine degradation, citric acid cycle (TCA cycle), and metabolism pathways where it interacts with FLII, ATP6V1B2, HSPD1, UMPS, and DDOST.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol DLST
Uniprot