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Dkk-4 Antibody

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Western Blot: Dkk-4 Antibody [NB100-1000] - (1ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot: Dkk-4 Antibody [NB100-1000] - (1ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, CaSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Dkk-4 Antibody Summary

Immunogen
Peptide with sequence RQHARLRVCQKIEKL corresponding to C-Terminus according to NP_055235.1.
Predicted Species
Mouse (100%), Rat (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DKK4
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
Application Notes
WB: Approx. 30-35 kDa band observed in Jurkat lysate. Please note that literature indicates DKK4 is detected as three major immunoreactive species of approx. 40 kDa band [form (i)], 30-32 kDa band [form (ii)] and 15-17 kDa band [form (iii)]. We believe will recognise 30-32 kDa band species of DKK4.
Control
COX-1 Overexpression Lysate
Publications
Read Publication using NB100-1000.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Dkk-4 Antibody

  • dickkopf (Xenopus laevis) homolog 4
  • dickkopf homolog 4 (Xenopus laevis)
  • dickkopf-4
  • dickkopf-related protein 4
  • Dkk4
  • Dkk-4
  • hDkk-4
  • MGC129562
  • MGC129563

Background

DKK4 is a secreted protein that is a member of the dickkopf family. Like some other members of the dickkopf family DKK4 contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The activity of DKK4 is modulated by binding to the Wnt co-receptor and the co-factor kremen 2, and it is proposed that it is also an inhibitor of the Wnt signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Dkk-4 Antibody (NB100-1000)(1)


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Product General Protocols

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Video Protocols

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FAQs for Dkk-4 Antibody (NB100-1000) (0)

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Control Lysate(s)

COX-1 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DKK4
Entrez
Uniprot