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Dkk-4 Antibody [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

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Dkk-4 Antibody [Biotin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Dkk-4 (R&D Systems, Catalog # 1269-DK)
Leu19-Leu224
Accession # NP_055235
Specificity
Detects human Dkk-4 in ELISAs and Western blots. In sandwich immunoassays, less than 2% cross-reactivity with recombinant mouse (rm) Dkk‑4 is observed and less than 0.3% cross-reactivity with recombinant human (rh) Dkk-1, rmDkk-1, rhDkk-3, and recombinant rat Dkk-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DKK4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Human Dkk-4 Antibody (Catalog # MAB1269)
ELISA Detection: Human Dkk-4 Biotinylated Antibody (Catalog # BAF1269)
Standard: Recombinant Human Dkk-4 (Catalog # 1269-DK)
Readout System

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dkk-4 Antibody [Biotin]

  • dickkopf (Xenopus laevis) homolog 4
  • dickkopf homolog 4 (Xenopus laevis)
  • dickkopf-4
  • dickkopf-related protein 4
  • Dkk4
  • Dkk-4
  • hDkk-4
  • MGC129562
  • MGC129563

Background

Dickkopf related protein 4 (Dkk-4) is a member of the Dkk protein family that includes Dkk-1, -2, -3, and -4 (1). All four members are secreted proteins that are synthesized as precursor proteins with an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region. Dkk proteins have potential furin type proteolytic cleavage sites, and short forms of Dkk-2 and Dkk-4 containing only the second cysteine-rich domain can be generated by proteolytic processing (1). Dkk proteins have distinct patterns of expression in adult and embryonic tissues, suggesting that they may play diverse roles in these tissues.

The Dkk proteins have distinct effects on Wnt signaling. Dkk-1 and Dkk-4 are Wnt antagonists. Dkk-3 has not been demonstrated to affect Wnt signaling, and Dkk-2 acts as an agonist or antagonist, depending on the cellular context. Wnt signaling regulates many important developmental processes including neural crest differentiation, brain development, kidney morphogenesis, and sex determination. In addition, Wnt signaling has also been implicated in tumor formation. Canonical Wnt signaling via the beta-catenin pathway is transduced by a receptor complex composed of the Frizzled proteins (Fz) and low-density lipoprotein (LDL) receptor-related proteins (LRP5/6) (2, 3). Unlike many soluble Wnt antagonists that function by binding extracellular Wnt ligands to prevent interaction of Wnt with the Fz‑LRP5/6 receptor complex, Dkk-1 and Dkk-4 antagonize Wnt/beta-catenin signaling by direct high-affinity binding to the Wnt coreceptor LRP5/6 and inhibiting interaction of LRP5/6 with the Wnt-Frizzled complex (4). Dkk-1 and Dkk-4 also bind the transmembrane proteins Kremen1 (Krm1) and Krm2 with high-affinity (5). Krm2 has been shown to form a ternary complex with Dkk-1 or -4 and LRP5/6 to trigger internalization of the complex and removal of LRP6 from the cell surface. Thus, Dkk-1/4 and Kremens function synergistically to antagonize LRP5/6-mediated Wnt activity. Dkk-2 also binds to LRP5/6 and the Kremens, but Dkk-2 acts as an antagonist of the Wnt signaling pathway only in the presence of Krm2 (5, 6). Dkk-2 binding to LRP5/6 in the absence of Krm2 activates rather than inhibits Wnt signalling (6).

  1. Krupnik, V.E. et al. (1999) Gene 238:301.
  2. Zorn, A.M. (2001) Current Biology R592.
  3. Mao, J. et al. (2001) Mol. Cell 7:801.
  4. Nusse, R. et al. (2001) Nature 411:255.
  5. Mao, J. et al. (2002) Nature 417:664.
  6. Mao, B. and C. Niehrs (2003) Gene 302:179.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol DKK4
Uniprot