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DHODH Antibody

Images

 
Western Blot: DHODH Antibody [NBP1-86097] - Analysis in human cell line MCF-7.
Immunocytochemistry/ Immunofluorescence: DHODH Antibody [NBP1-86097] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & mitochondria.
Immunohistochemistry-Paraffin: DHODH Antibody [NBP1-86097] - Staining of human placenta shows strong cytoplasmic granular positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: DHODH Antibody [NBP1-86097] - Staining of human kidney shows strong cytoplasmic positivity in granular pattern in tubule cells.
Immunohistochemistry-Paraffin: DHODH Antibody [NBP1-86097] - Staining of human colon shows moderate cytoplasmic granular positivity in glandular cells.
Immunohistochemistry-Paraffin: DHODH Antibody [NBP1-86097] - Staining of human liver shows moderate cytoplasmic granular positivity in hepatocytes.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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DHODH Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LAVRFTSLGLLPRARFQDSDMLEVRVLGHKFRNPVGIAAGFDKHGEAVDGLYKMGFGFVEIGSVTPKPQEGNPRPRVFRLPEDQAVINRYGFNSHGLSVVEHRLRG
Predicted Species
Mouse (92%), Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DHODH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20-1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
DHODH Protein (NBP1-86097PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DHODH Antibody

  • DHOdehase
  • DHODH
  • dihydroorotate dehydrogenase
  • dihydroorotate dehydrogenase, mitochondrial
  • Dihydroorotate Oxidase
  • EC 1.3.3.1
  • EC 1.3.5.2
  • human complement of yeast URA1
  • POADS
  • URA1

Background

DHODH is encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol DHODH