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UMPS Antibody

Images

 
Genetic Strategies: Western Blot: UMPS Antibody [NBP1-85896] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Immunocytochemistry/ Immunofluorescence: UMPS Antibody [NBP1-85896] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: UMPS Antibody [NBP1-85896] - Staining of human prostate shows weak to moderate cytoplasmic positivity in glandular cells.
Western Blot: UMPS Antibody [NBP1-85896] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunohistochemistry-Paraffin: UMPS Antibody [NBP1-85896] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: UMPS Antibody [NBP1-85896] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: UMPS Antibody [NBP1-85896] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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UMPS Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GLKVTDAIVLLDREQGGKDKLQAHGIRLHSVCTLSKMLEILEQQKKVDAETVGRVKRFIQENVFVAANHNASPLSIKEAPKELSFGARAELPRIHPVA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UMPS
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
UMPS Protein (NBP1-85896PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for UMPS Antibody

  • EC 4.1.1.23
  • OMPdecase
  • OPRT
  • OPRTase
  • orotate phosphoribosyltransferase
  • orotidine 5'-phosphate decarboxylase
  • UMP synthase
  • uridine 5'-monophosphate synthase
  • uridine monophosphate synthetase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for UMPS Antibody (NBP1-85896) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol UMPS