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DHCR7 Antibody - Azide and BSA Free

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Western Blot: DHCR7 Antibody [NBP3-04976] - Western blot analysis of extracts of various cell lines, using DHCR7 antibody (NBP3-04976) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free

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Catalog# & Formulation Size Price
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DHCR7 Antibody - Azide and BSA Free Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 346-475 of human DHCR7 (NP_001351.2). VGYYIFRVANHQKDLFRRTDGRCLIWGRKPKVIECSYTSADGQRHHSKLLVSGFWGVARHFNYVGDLMGSLAYCLACGGGHLLPYFYIIYMAILLTHRCLRDEHRCASKYGRDWERYTAAVPYRLLPGIF
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DHCR7
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.05% Proclin 300
Purity
Affinity purified

Alternate Names for DHCR7 Antibody - Azide and BSA Free

  • 7-dehydrocholesterol reductase
  • 7-DHC reductase
  • D7SR
  • delta-7-dehydrocholesterol reductase
  • EC 1.3.1
  • EC 1.3.1.21
  • Putative sterol reductase SR-2
  • SLOS
  • Smith-Lemli-Opitz syndrome
  • Sterol Delta(7)-reductase
  • sterol delta-7-reductase

Background

The DHCR7 gene encodes delta-7-sterol reductase (EC 1.3.1.21), the penultimate enzyme of mammalian sterol biosynthesis that converts 7-dehydrocholesterol (7-DHC) to cholesterol. This enzyme removes the C(7-8) double bond introduced by the sterol delta8-delta7 isomerases. In addition, its role in drug-induced malformations is known: inhibitors of the last step of cholesterol biosynthesis such as AY9944 and BM15766 severely impair brain development (Moebius et al., 1998 [PubMed 9465114]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DHCR7