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Desmocollin-1 Antibody (772906) [Unconjugated]

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Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line and B16-F1 mouse melanoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rat Anti-Human/Mouse Desmocollin-1 Monoclonal Antibody (Catalog # MAB7367) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Desmocollin-1 at approximately 98 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line and B16-F1 mouse melanoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rat Anti-Human/Mouse Desmocollin-1 Monoclonal Antibody (Catalog ...read more
B16‑F1 mouse melanoma cell line was stained with Rat Anti-Human/Mouse Desmocollin‑1 Monoclonal Antibody (Catalog # MAB7367, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed ...read more
A549 human lung carcinoma cell line was stained with Rat Anti-Human/Mouse Desmocollin‑1 Monoclonal Antibody (Catalog # MAB7367, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), ...read more
Desmocollin-1 was detected in immersion fixed A549 human lung carcinoma cell line using Rat Anti-Human/Mouse Desmocollin-1 Monoclonal Antibody (Catalog # MAB7367) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more
Desmocollin-1 was detected in perfusion fixed frozen sections of mouse skin using Rat Anti-Human/Mouse Desmocollin-1 Monoclonal Antibody (Catalog # MAB7367) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready, ICC/IF
Clone
772906
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB7367U-100UG
Alexa Fluor 405
FAB7367V-100UG
Alexa Fluor 488
FAB7367G-100UG
Alexa Fluor 532
FAB7367X
Alexa Fluor 594
FAB7367T-100UG
Alexa Fluor 647
FAB7367R-100UG
Alexa Fluor 700
FAB7367N-100UG
Alexa Fluor 750
FAB7367S-100UG
Allophycocyanin/Cy7
FAB7367APCCY7
Biotin
FAB7367B
CoraFluor 1
FAB7367CL1
DyLight 350
FAB7367D
DyLight 405
FAB7367E
DyLight 488
FAB7367K
DyLight 550
FAB7367L
DyLight 594
FAB7367M
DyLight 650
FAB7367W
DyLight 680
FAB7367Y
DyLight 755
FAB7367Z
FITC
FAB7367F
HRP
FAB7367H
Janelia Fluor 525
FAB7367JF525
Janelia Fluor 549
FAB7367I
Janelia Fluor 646
FAB7367J
Janelia Fluor 669
FAB7367JF669
PE
FAB7367P
PE/Atto594
FAB7367PEATT594
PE/Cy5.5
FAB7367PECY55
PE/Cy7
FAB7367PECY7
PerCP
FAB7367C
mFluor Violet 450 SE
FAB7367MFV450
mFluor Violet 500 SE
FAB7367MFV500
mFluor Violet 610 SE
FAB7367MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB7367-SP
Carrier Free
FAB7367V-100UG
Carrier Free
FAB7367U-100UG
Carrier Free
FAB7367T-100UG
Carrier Free
FAB7367S-100UG
Carrier Free
FAB7367R-100UG
Carrier Free
FAB7367N-100UG
Carrier Free
FAB7367G-100UG
Novus Biologicals offers 100% Guarantee

Desmocollin-1 Antibody (772906) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Desmocollin-1
Arg135-Lys691
Accession # P55849
Specificity
Detects mouse Desmocollin-1 in ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human Desmocollin-1 is observed, and no cross-reactivity with recombinant mouse Desmocollin-2 or -3 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
DSC1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 1-25 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
MAB7367 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Desmocollin-1 Antibody (772906) [Unconjugated]

  • cadherin family member 1
  • CDHF1
  • desmocollin 1
  • Desmocollin1
  • Desmocollin-1
  • desmosomal glycoprotein 2/3
  • DG2/DG3
  • DG2/DG3
  • DSC1

Background

DSC-1 (Desmocollin [Greek for "glue-that-binds"]-1) is an approximately 95-110 kDa member of the Ca++-dependent cadherin family of adhesion molecules. It is found on the surface of stratified epithelial cells, including the spinous and granular layers of keratinized and nonkeratinized epithelia of the oral cavity and skin.
DSC‑1 expression is induced by DSG-1. It serves as a component of desmosomes, forming a linkage that unites adjacent cells with cytoplasmic intermediate filaments. In particular, homodimeric DSC-1 may form heterotypic interactions with DSG-1 in-trans, and bind to the cytoskeleton intracellularly via plakophilin-1. Mature mouse DSC-1 is a 760 amino acid (aa) type I transmembrane glycoprotein (aa 135-691). The mature molecule contains a 557 aa extracellular region with five cadherin domains (aa 135-682), and a 172 aa cytoplasmic domain. There is one splice variant that shows an 11 aa substitution for aa 822-886. Over aa 135-691, mouse DSC‑1 shares 82% aa sequence identity with human DSC-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Desmocollin-1 Antibody (MAB7367)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC-Fr, Western Blot.


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(1)
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Bioinformatics

Gene Symbol DSC1
Uniprot