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Dcp1a Antibody

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Immunocytochemistry/Immunofluorescence: Dcp1a Antibody [NBP2-59785] - Human cell line U-2 OS shows localization to cytoplasmic bodies. Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: Dcp1a Antibody [NBP2-59785] - Human cell line U-2 OS shows localization to cytoplasmic bodies. Antibody staining is shown in green.
Dcp1a Antibody [NBP2-59785] -Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.
Dcp1a Antibody [NBP2-59785] - Staining of human pancreas shows moderate to strong cytoplasmic positivity in exocrine glandular cells.
Dcp1a Antibody [NBP2-59785] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.
Dcp1a Antibody [NBP2-59785] - Staining of human skeletal muscle shows weak cytoplasmic positivity in myocytes.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Dcp1a Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acid sequence: RSPLLNQPVPELSHASLIANQSPFRAPLNVTNTAGTSLPSVDLLQKLRLTPQHDQIQTQPLGKGAMVASFSPAAGQLATPESFIEPPSKTAAARVAASASLSNMVLAPLQSMQQNQDPEVFVQPKVLSSASA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DCP1A
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Dcp1a Recombinant Protein Antigen (NBP2-59785PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (82%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Dcp1a Antibody

  • DCP1 decapping enzyme homolog A (S. cerevisiae)
  • decapping enzyme hDcp1a
  • EC 3.-
  • FLJ21691
  • mRNA-decapping enzyme 1A
  • Nbla00360
  • putative protein product of Nbla00360
  • Smad4-interacting transcriptional co-activator
  • SMAD4IP1
  • SMIFHSA275986
  • Transcription factor SMIF

Background

Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol DCP1A