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DC-SIGNR/CD299/CLEC4M Antibody (120604) [Alexa Fluor® 750]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
120604
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 750
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB162U-100UG
Alexa Fluor 405
FAB162V-100UG
Alexa Fluor 488
FAB162G-100UG
Alexa Fluor 532
FAB162X
Alexa Fluor 594
FAB162T-100UG
Alexa Fluor 647
FAB162R-100UG
Alexa Fluor 700
FAB162N-100UG
Allophycocyanin
FAB162A
Allophycocyanin/Cy7
FAB162APCCY7
Biotin
FAB162B
DyLight 350
FAB162D
DyLight 405
FAB162E
DyLight 488
FAB162K
DyLight 550
FAB162L
DyLight 594
FAB162M
DyLight 650
FAB162W
DyLight 680
FAB162Y
DyLight 755
FAB162Z
HRP
FAB162H
Janelia Fluor 549
FAB162I
Janelia Fluor 646
FAB162J
PE/Atto594
FAB162PEATT594
PE/Cy5.5
FAB162PECY55
PE/Cy7
FAB162PECY7
PerCP
FAB162C
Phycoerythrin
FAB162P
Unconjugated
MAB162
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB162
Carrier Free
FAB162P
Carrier Free
MAB162
Carrier Free
MAB162-SP
Carrier Free
FAB162V-100UG
Carrier Free
FAB162U-100UG
Carrier Free
FAB162T-100UG
Carrier Free
FAB162R-100UG
Carrier Free
FAB162N-100UG
Carrier Free
FAB162G-100UG
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DC-SIGNR/CD299/CLEC4M Antibody (120604) [Alexa Fluor® 750] Summary

Additional Information
Clone 120604 was used by HLDA to establish CD designation
Immunogen
NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC‑SIGNR/CD299
Accession # Q9H2X3
Specificity
Detects human DC‑SIGNR/CD299.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CLEC4M
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DC-SIGNR/CD299/CLEC4M Antibody (120604) [Alexa Fluor® 750]

  • CD209L
  • CD209L1
  • CD209Ldendritic cell-specific ICAM-3-grabbing nonintegrin 2
  • CD299 antigenMGC129964
  • CD299
  • CLEC4M
  • C-type lectin domain family 4, member M
  • DC-SIGN2
  • DC-SIGN2CD209 antigen-like protein 1
  • DCSIGNR
  • DC-SIGNR
  • DC-SIGNRDC-SIGN-related protein
  • DCSIGNRMGC47866
  • Dendritic cell-specific ICAM-3-grabbing non-integrin 2
  • HP10347
  • liver/lymph node-specific ICAM-3 grabbing non-integrin
  • Liver/lymph node-specific ICAM-3-grabbing non-integrin
  • LSIGN
  • L-SIGN
  • LSIGNC-type lectin domain family 4 member M
  • mannose binding C-type lectin DC-SIGNR

Background

Dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN or CD299) and DC-SIGN related protein (DC-SIGNR, DC-SIGN2, L-SIGN or CD209L) are type II membrane proteins that are mannose-specific calcium-dependent (C-type) lectins. The two proteins share 77% amino acid identity. DC-SIGN mediates interactions between dendritic cells (DCs) and T cells. Both DC-SIGN and DC-SIGNR have been shown to bind HIV, hepatitis C glycoproteins, Ebola virus glycoproteins and the cellular adhesion protein ICAM-3 (1‑4). DC-SIGN and DC-SIGNR appear to selectively recognize and bind viral proteins containing a large portion of high-mannose oligosaccharides (5). Though DC-SIGN and DC-SIGNR are found on the same chromosome, they are not expressed in the same tissue. DC-SIGN is expressed solely on Dendritic cells while DC-SIGNR is found on endothelial cells in the liver and lymph node sinuses and in a significant portion of capillary endothelial cells in term placenta (1, 4).

  1. Pohlmann, S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:2670.
  2. Pohlmann, S. et al. (2003) J. Virol. 77:4070.
  3. Simmons, L.G. et al. (2003) J. Virol. 77:1337.
  4. Bahirova, A.A. et al. (2001) J. Exp. Med. 193:671.
  5. Feinberg, H. et al. (2001) Science 294:2163.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CLEC4M
Uniprot