Home » DC-SIGNR/CD299/CLEC4M » DC-SIGNR/CD299/CLEC4M Antibodies » DC-SIGNR/CD299/CLEC4M Antibody (120604) [Phycoerythrin]
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DC-SIGNR/CD299/CLEC4M Antibody (120604) [Phycoerythrin]

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3T3 mouse embryonic fibroblast cell line transfected with human DC-SIGNR/CD299 was stained with Mouse Anti-Human DC-SIGNR/CD299 PE-conjugated Monoclonal Antibody (Catalog # FAB162P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.
3T3 mouse embryonic fibroblast cell line transfected with human DC-SIGNR/CD299 was stained with Mouse Anti-Human DC-SIGNR/CD299 PE-conjugated Monoclonal Antibody (Catalog # FAB162P, filled histogram) or isotype control ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
120604
Clonality
Monoclonal
Host
Mouse
Conjugate
Phycoerythrin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB162U-100UG
Alexa Fluor 405
FAB162V-100UG
Alexa Fluor 488
FAB162G-100UG
Alexa Fluor 532
FAB162X
Alexa Fluor 594
FAB162T-100UG
Alexa Fluor 647
FAB162R-100UG
Alexa Fluor 700
FAB162N-100UG
Alexa Fluor 750
FAB162S-100UG
Allophycocyanin
FAB162A
Allophycocyanin/Cy7
FAB162APCCY7
Biotin
FAB162B
DyLight 350
FAB162D
DyLight 405
FAB162E
DyLight 488
FAB162K
DyLight 550
FAB162L
DyLight 594
FAB162M
DyLight 650
FAB162W
DyLight 680
FAB162Y
DyLight 755
FAB162Z
HRP
FAB162H
Janelia Fluor 525
FAB162JF525
Janelia Fluor 549
FAB162I
Janelia Fluor 585
FAB162JF585
Janelia Fluor 635
FAB162JF635
Janelia Fluor 646
FAB162J
Janelia Fluor 669
FAB162JF669
PE/Atto594
FAB162PEATT594
PE/Cy5.5
FAB162PECY55
PE/Cy7
FAB162PECY7
PerCP
FAB162C
Unconjugated
MAB162
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB162
Carrier Free
MAB162
Carrier Free
MAB162-SP
Carrier Free
FAB162V-100UG
Carrier Free
FAB162U-100UG
Carrier Free
FAB162T-100UG
Carrier Free
FAB162S-100UG
Carrier Free
FAB162R-100UG
Carrier Free
FAB162N-100UG
Carrier Free
FAB162G-100UG
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DC-SIGNR/CD299/CLEC4M Antibody (120604) [Phycoerythrin] Summary

Additional Information
Clone 120604 was used by HLDA to establish CD designation
Immunogen
NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC‑SIGNR/CD299
Accession # Q9H2X3
Specificity
Detects human DC‑SIGNR/CD299.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CLEC4M
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
FAB162P in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DC-SIGNR/CD299/CLEC4M Antibody (120604) [Phycoerythrin]

  • CD209L
  • CD209L1
  • CD209Ldendritic cell-specific ICAM-3-grabbing nonintegrin 2
  • CD299 antigenMGC129964
  • CD299
  • CLEC4M
  • C-type lectin domain family 4, member M
  • DC-SIGN2
  • DC-SIGN2CD209 antigen-like protein 1
  • DCSIGNR
  • DC-SIGNR
  • DC-SIGNRDC-SIGN-related protein
  • DCSIGNRMGC47866
  • Dendritic cell-specific ICAM-3-grabbing non-integrin 2
  • HP10347
  • liver/lymph node-specific ICAM-3 grabbing non-integrin
  • Liver/lymph node-specific ICAM-3-grabbing non-integrin
  • LSIGN
  • L-SIGN
  • LSIGNC-type lectin domain family 4 member M
  • mannose binding C-type lectin DC-SIGNR

Background

Dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN or CD299) and DC-SIGN related protein (DC-SIGNR, DC-SIGN2, L-SIGN or CD209L) are type II membrane proteins that are mannose-specific calcium-dependent (C-type) lectins. The two proteins share 77% amino acid identity. DC-SIGN mediates interactions between dendritic cells (DCs) and T cells. Both DC-SIGN and DC-SIGNR have been shown to bind HIV, hepatitis C glycoproteins, Ebola virus glycoproteins and the cellular adhesion protein ICAM-3 (1-4). DC-SIGN and DC-SIGNR appear to selectively recognize and bind viral proteins containing a large portion of high-mannose oligosaccharides (5). Though DC-SIGN and DC-SIGNR are found on the same chromosome, they are not expressed in the same tissue. DC-SIGN is expressed solely on Dendritic cells while DC-SIGNR is found on endothelial cells in the liver and lymph node sinuses and in a significant portion of capillary endothelial cells in term placenta (1, 4).

  1. Pohlmann, S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:2670.
  2. Pohlmann, S. et al. (2003) J. Virol. 77:4070.
  3. Simmons, L.G. et al. (2003) J. Virol. 77:1337.
  4. Bahirova, A.A. et al. (2001) J. Exp. Med. 193:671.
  5. Feinberg, H. et al. (2001) Science 294:2163.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DC-SIGNR/CD299/CLEC4M Antibody (FAB162P)(4)

We have publications tested in 2 confirmed species: Human, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 1 application: Flow Cytometry.


Filter By Application
Flow Cytometry
(4)
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Human
(4)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
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Showing Publications 1 - 4 of 4.
Publications using FAB162P Applications Species
KM Bertram, RA Botting, H Baharlou, JW Rhodes, H Rana, JD Graham, E Patrick, J Fletcher, TM Plasto, NR Truong, C Royle, CM Doyle, O Tong, N Nasr, L Barnouti, MP Kohout, AJ Brooks, MP Wines, P Haertsch, J Lim, MP Gosselink, G Ctercteko, JD Estes, MJ Churchill, PU Cameron, E Hunter, MA Haniffa, AL Cunningham, AN Harman Identification of HIV transmitting CD11c+ human epidermal dendritic cells Nat Commun, 2019-06-21;10(1):2759. 2019-06-21 [PMID: 31227717] (Flow Cytometry, Human) Flow Cytometry Human
RA Botting, KM Bertram, H Baharlou, KJ Sandgren, J Fletcher, JW Rhodes, H Rana, TM Plasto, XM Wang, JJ Lim, L Barnouti, MP Kohout, T Papadopoul, S Merten, N Olbourne, AL Cunningham, M Haniffa, AN Harman Phenotypic and functional consequences of different isolation protocols on skin mononuclear phagocytes J. Leukoc. Biol, 2017-03-07;0(0):. 2017-03-07 [PMID: 28270408] (Flow Cytometry, Human) Flow Cytometry Human
Mukherjee S, Dowd K, Manhart C, Ledgerwood J, Durbin A, Whitehead S, Pierson T Mechanism and significance of cell type-dependent neutralization of flaviviruses. J Virol, 2014-04-16;88(13):7210-20. 2014-04-16 [PMID: 24741083] (Flow Cytometry, Human) Flow Cytometry Human
Messmer D, Jacque JM, Santisteban C, Bristow C, Han SY, Villamide-Herrera L, Mehlhop E, Marx PA, Steinman RM, Gettie A, Pope M Endogenously expressed nef uncouples cytokine and chemokine production from membrane phenotypic maturation in dendritic cells. J. Immunol., 2002-10-15;169(8):4172-82. 2002-10-15 [PMID: 12370346] (Flow Cytometry, Human, Primate - Macaca mulatta (Rhesus Macaque)) Flow Cytometry Human, Primate - Macaca mulatta (Rhesus Macaque)

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Bioinformatics

Gene Symbol CLEC4M
Uniprot