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DAZ1 Antibody (3A4)

Images

 
Western Blot: DAZ1 Antibody (3A4) [H00001617-M09] - DAZ1 monoclonal antibody (M09), clone 3A4. Western Blot analysis of DAZ1 expression in human placenta.
Immunohistochemistry-Paraffin: DAZ1 Antibody (3A4) [H00001617-M09] - Analysis of monoclonal antibody to DAZ1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml
ELISA: DAZ1 Antibody (3A4) [H00001617-M09] - Detection limit for recombinant GST tagged DAZ1 is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clone
3A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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DAZ1 Antibody (3A4) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
DAZ1 (AAH18119, 21 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SSSAAASQGWVLPEGKIVPNTVFVGGIDARMDETEIGSCFGRYGSVKEVKIITNRTGVSKGYGFVSFVNDVDVQKIVGSQIHFHGKKLKLGPAIRKQKLC
Specificity
DAZ1 - deleted in azoospermia 1
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DAZ1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
Application Notes
Antibody reactivity against tissue lysate and recombinant protein for WB. It has been used for IHC-P and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DAZ1 Antibody (3A4)

  • deleted in azoospermia 1
  • deleted in azoospermia
  • FLJ17175
  • SPGYDAZdeleted in azoospermia protein 1

Background

This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains three copies of the 10.8 kb repeat. However, no transcripts containing three copies of the RRM domain have been described; thus the RefSeq for this gene contains only two RRM domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol DAZ1
Entrez
OMIM
Uniprot