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Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody

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Western Blot: Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody [NBP2-32596] - Analysis in human cell line CAPAN-2
Immunocytochemistry/ Immunofluorescence: Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody [NBP2-32596] - Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Immunohistochemistry-Paraffin: Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody [NBP2-32596] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.
Independent Antibodies: Staining of human lymph node, prostate, skin and small intestine using NBP2-32596 (A) shows similar protein distribution across tissues to independent antibody NBP2-33396 (B).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

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Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: HIKGWLRMKGKDNFLFITYEELQQDLQGSVERICGFLGRPLGKEALGSVVAHSTFSAMKANTMSNYTLLPPSLLDHRR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SULT2B1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Cytosolic Sulfotransferase 2B1/SULT2B1 Protein (NBP2-32596PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (81%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody

  • Alcohol sulfotransferase
  • Cytosolic Sulfotransferase 2B1
  • EC 2.8.2
  • EC 2.8.2.2
  • HSST2
  • HSST2sulfotransferase family cytosolic 2B member 1
  • Hydroxysteroid sulfotransferase 2
  • ST2B1
  • Sulfotransferase 2B1
  • sulfotransferase family, cytosolic, 2B, member 1
  • SULT2B1

Background

Sulfotransferase family cytosolic 2B member 1 isoform b, also known as SULT2B1, is a member of Sulfotransferase family. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. SULT2B1b is localized in the cytosol and nuclei of human cells. SULT2B1b is selective for the sulfation of 3beta-hydroxysteroids such as dehydroepiandrosterone and pregnenolone, and may also have a role in cholesterol sulfation in human skin. Recombinant human SULT2B1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SULT2B1