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Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (726211)

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Western blot shows lysates of human liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Cytosolic Sulfotransferase 1E1/SULT1E1 Monoclonal Antibody (Catalog # MAB5545) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cytosolic Sulfotransferase 1E1/SULT1E1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Cytosolic Sulfotransferase 1E1/SULT1E1 Monoclonal Antibody (Catalog # MAB5545) followed by HRP-conjugated ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
726211
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB5545-SP
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Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (726211) Summary

Immunogen
E. coli-derived recombinant human Cytosolic Sulfotransferase 1E1/SULT1E1
Asn2-Ile294
Accession # P49888
Specificity
Detects human Cytosolic Sulfotransferase 1E1/SULT1E1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human SULT1A1, 1B1, 2A1, 2B1, or 4A1 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SULT1E1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB5545 in the following application:
Publications
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MAB5545 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (726211)

  • Cytosolic Sulfotransferase 1E1
  • EC 2.8.2
  • EST-1
  • ESTMGC34459
  • estrone sulfotransferase
  • ST1E1
  • STE
  • STEestrogen sulfotransferase
  • Sulfotransferase 1E1
  • sulfotransferase family 1E, estrogen-preferring, member 1
  • Sulfotransferase, estrogen-preferringEC 2.8.2.4
  • SULT1E1

Background

Cytosolic Sulfotransferases catalyze the sulfation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm. SULT1E1 is widely known as an estrogen sulfotransferase and may control estrogen levels by converting free estradiol to its inactive sulfate conjugate (1). Known substrates of this enzyme also include dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol, 4‑nitrophenol, 1-napthol and resveratrol (2, 3). SULT1E1 activity is found in reproductive organs (4), intestine and liver (3, 5).
  1. Gong, H. et al. (2008) Cancer Res. 68:7386.
  2. Falany, C.N. et al. (1995) J. Steroid Biochem. Mol. Biol. 52:529.
  3. Miksits, M. et al. 2005, Xenobiotica 35:1101.
  4. Takase, Y. et al. (2007) The Prostate 67:405.
  5. Schrag, M.L. et al. (2004) Drug Metabolism and Disposition 32:1299.
  6. Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, 964.
  7. MacRae, I.J., I.H. Segel, and A.J. Fisher. (2000) Biochemistry. 39: 1613.
  8. Wu, Z.L., et al. (2002) Faseb J. 16:539.
    9.  

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (MAB5545)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Simple Western.


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Review for Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (MAB5545) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human and Mouse.

Reviews using MAB5545:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Hua Jiang
 WB Human and Mouse 08/16/2021
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Summary

ApplicationWestern Blot
Sample Testedbrain and spinal cord,Liver,Mouse brain
SpeciesHuman and Mouse
LotCGNT012107A

Comments

Comments***Novus Innovators Program - new species or application used on a primary antibody.

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Recent Reviews

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Western Blot Cytosolic Sulfotransferase 1E1/SULT1E1 MAB5545
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 Hua Jiang
08/16/2021
Application: WB
Species: Human and Mouse
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Bioinformatics

Gene Symbol SULT1E1
Uniprot