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Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1)

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Western Blot: GBA3 Antibody (1F1) [NBP2-00521] Analysis of extracts (35ug) from 9 different cell lines by using anti-GBA3 monoclonal antibody.
Western Blot: GBA3 Antibody (1F1) [NBP2-00521] Analysis of extracts (35ug) from 9 different cell lines by using anti-GBA3 monoclonal antibody.
Immunohistochemistry: Cytosolic beta-Glucosidase/GBA3 Antibody (1F1) [NBP2-00521] - GBA3 Antibody (1F1) [NBP2-00521] - Staining of paraffin-embedded Human prostate tissue using anti-GBA3 mouse monoclonal antibody.
Flow Cytometry: GBA3 Antibody (1F1) [NBP2-00521] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GBA3 antibody, and then analyzed by ...read more
Western Blot: GBA3 Antibody (1F1) [NBP2-00521] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBA3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunohistochemistry-Paraffin: GBA3 Antibody (1F1) [NBP2-00521] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GBA3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GBA3 Antibody (1F1) [NBP2-00521] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GBA3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GBA3 Antibody (1F1) [NBP2-00521] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GBA3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GBA3 Antibody (1F1) [NBP2-00521] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-GBA3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GBA3 Antibody (1F1) [NBP2-00521] - Staining of paraffin-embedded Human liver tissue using anti-GBA3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GBA3 Antibody (1F1) [NBP2-00521] - Staining of paraffin-embedded Human lymphoma tissue using anti-GBA3 mouse monoclonal antibody.
Flow Cytometry: GBA3 Antibody (1F1) [NBP2-00521] - Analysis of Hela cells, using anti-GBA3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: GBA3 Antibody (1F1) [NBP2-00521] - Analysis of Jurkat cells, using anti-GBA3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC
Clone
OTI1F1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-72100AF350
Alexa Fluor 405
NBP2-72100AF405
Alexa Fluor 488
NBP2-72100AF488
Alexa Fluor 532
NBP2-72100AF532
Alexa Fluor 594
NBP2-72100AF594
Alexa Fluor 647
NBP2-72100AF647
Alexa Fluor 700
NBP2-72100AF700
Alexa Fluor 750
NBP2-72100AF750
Allophycocyanin
NBP2-72100APC
Allophycocyanin/Cy7
NBP2-72100APCCY7
Biotin
NBP2-72100B
DyLight 350
NBP2-72100UV
DyLight 405
NBP2-72100V
DyLight 488
NBP2-72100G
DyLight 550
NBP2-72100R
DyLight 594
NBP2-72100DL594
DyLight 650
NBP2-72100C
DyLight 680
NBP2-72100FR
DyLight 755
NBP2-72100IR
FITC
NBP2-72100F
HRP
NBP2-72100H
Janelia Fluor 549
NBP2-72100JF549
Janelia Fluor 646
NBP2-72100JF646
PE
NBP2-72100PE
PE/Atto594
NBP2-72100PEATT594
PE/Cy5.5
NBP2-72100PECY55
PE/Cy7
NBP2-72100PECY7
PerCP
NBP2-72100PCP
mFluor Violet 450 SE
NBP2-72100MFV450
mFluor Violet 500 SE
NBP2-72100MFV500
mFluor Violet 610 SE
NBP2-72100MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-72100
100% Guarantee on every product

Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 1-150 and 370-469 of human GBA3 (NP_066024) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
GBA3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
53.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1)

  • CBG
  • CBGL1
  • CBGL1GLUC
  • Cytosolic betaGlucosidase
  • Cytosolic beta-Glucosidase
  • Cytosolic beta-glucosidase-like protein 1
  • EC 3.2.1.21
  • GBA3
  • GLUC
  • glucosidase, beta, acid 3 (cytosolic)
  • klotho-related protein
  • KLrP
  • MGC104276
  • MGC126878

Background

GBA3, or cytosolic beta-glucosidase (EC 3.2.1.21), is a predominantly liver enzyme that efficiently hydrolyzes beta-D-glucoside and beta-D-galactoside, but not any known physiologic beta-glycoside, suggesting that it may be involved in detoxification of plant glycosides (de Graaf et al., 2001 [PubMed 11389701]). GBA3 also has significant neutral glycosylceramidase activity (EC 3.2.1.62), suggesting that it may be involved in a nonlysosomal catabolic pathway of glucosylceramide metabolism (Hayashi et al., 2007 [PubMed 17595169]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-00521
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Bioinformatics

Gene Symbol GBA3
Entrez