![Western Blot: Cytokeratin 18 Antibody (DC10) - Azide and BSA Free [NBP2-33082] - Western Blot Analysis of human Intestine tissue lysate using Cytokeratin 18 Antibody (DC10).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DC10-Azide-and-BSA-Free-Western-Blot-NBP2-33082-img0007.jpg "Western Blot: Cytokeratin 18 Antibody (DC10) - Azide and BSA Free [NBP2-33082] - Western Blot Analysis of human Intestine tissue lysate using Cytokeratin 18 Antibody (DC10).")
| Reactivity | Hu, Ma(-), Bv(-), Ca(-), Ha(-), Mu(-), Po(-), Rt(-), Sh(-)Species Glossary |
| Applications | WB, Simple Western, Flow, ICC/IF, IHC |
| Clone | DC10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-29461). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | PMC-42 human breast carcinoma cells |
| Localization | Cytoplasmic |
| Marker | Epithelial Marker |
| Specificity | This monoclonal antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT18 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size. |
| Theoretical MW | 45 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 18 Antibody (NBP2-33082)Find related products by research area.
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Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen... Read full blog post. |
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| Gene Symbol | KRT18 |
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DC10) - Azide and BSA Free [NBP2-33082] - Staining of human skin sweat gland. Note cytoplasmic staining of tumor cells.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DC10-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33082-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DC10) - Azide and BSA Free [NBP2-33082] - Staining of human skin sweat gland. Note cytoplasmic staining of tumor cells.")
![Western Blot: Cytokeratin 18 Antibody (DC10) - Azide and BSA Free [NBP2-33082] - Analysis of Keratin 18 in 1) HeLa and 2) A431 lysate using Keratin 18 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DC10-Azide-and-BSA-Free-Western-Blot-NBP2-33082-img0003.jpg "Western Blot: Cytokeratin 18 Antibody (DC10) - Azide and BSA Free [NBP2-33082] - Analysis of Keratin 18 in 1) HeLa and 2) A431 lysate using Keratin 18 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: Cytokeratin 18 Antibody (DC10) - Azide and BSA Free [NBP2-33082] - Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Antibody (DC10).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DC10-Azide-and-BSA-Free-Western-Blot-NBP2-33082-img0006.jpg "Western Blot: Cytokeratin 18 Antibody (DC10) - Azide and BSA Free [NBP2-33082] - Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Antibody (DC10).")
![Simple Western: Cytokeratin 18 Antibody (DC10) - Azide and BSA Free [NBP2-33082] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 18 antibody was used at 10 ug/ml dilution of HeLa lysates(s) respectively.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DC10-Azide-and-BSA-Free-Simple-Western-NBP2-33082-img0004.jpg "Simple Western: Cytokeratin 18 Antibody (DC10) - Azide and BSA Free [NBP2-33082] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 18 antibody was used at 10 ug/ml dilution of HeLa lysates(s) respectively.")
![Simple Western: Cytokeratin 18 Antibody (DC10) - Azide and BSA Free [NBP2-33082] - Simple Western lane view shows a specific band for Cytokeratin 18 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DC10-Azide-and-BSA-Free-Simple-Western-NBP2-33082-img0005.jpg "Simple Western: Cytokeratin 18 Antibody (DC10) - Azide and BSA Free [NBP2-33082] - Simple Western lane view shows a specific band for Cytokeratin 18 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
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