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Cytokeratin 18 Antibody (C-04) [Biotin]

Images

 
Western Blot: Cytokeratin 18 Antibody (C-04) [Biotin] [NB500-640] - Analysis of cytokeratin 18 in HeLa cells with anti-cytokeratin 18 (C-04) biotin.
Immunohistochemistry: Cytokeratin 18 Antibody (C-04) [Biotin] [NB500-640] - Immunohistochemistry staining of human colon (paraffin-embedded sections) with anti-cytokeratin 18 (C-04).
Flow Cytometry: Cytokeratin 18 Antibody (C-04) [Biotin] [NB500-640] - Intracellular flow cytometry analysis of cytokeratin expression in HeLa human cervix carcinoma cell line using anti-cytokeratin 18 (C-04) FITC. ...read more
Immunohistochemistry: Cytokeratin 18 Antibody (C-04) [Biotin] [NB500-640] - Immunohistochemistry staining of human kidney (paraffin-embedded sections) with anti-cytokeratin 18 (C-04).

Product Details

Summary
Reactivity Hu, Mu, MaSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP, ICC/IF
Clone
C-04
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin
Concentration
1.0 mg/ml

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Cytokeratin 18 Antibody (C-04) [Biotin] Summary

Immunogen
Cytoskeleton preparation of epidermal carcinoma cell line A431.
Marker
Epithelial Marker
Specificity
The antibody C-04 reacts with Cytokeratin 18 (45 kDa), a member of intermediate filaments subfamily represented in epithelial tissues.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KRT18
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow (Intracellular) 1-4 ug/ml
  • Flow Cytometry 1-4 ug/ml
  • Immunocytochemistry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1-2 ug/ml
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Readout System
Control
Cytokeratin 18 Overexpression Lysate

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Cytokeratin 18 Antibody (C-04) [Biotin]

  • Cell proliferation-inducing gene 46 protein
  • cell proliferation-inducing protein 46
  • CK-18
  • CYK18
  • Cytokeratin 18
  • cytokeratin-18
  • K18
  • Keratin 18
  • keratin, type I cytoskeletal 18
  • keratin-18
  • KRT18

Background

Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratins 18 belongs to type I family (acidic cytokeratins).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cytokeratin 18 Antibody (NB500-640) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Research Areas for Cytokeratin 18 Antibody (NB500-640)

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Blogs on Cytokeratin 18.

Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component
Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

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Bioinformatics

Gene Symbol KRT18