![Western Blot: Cytokeratin 18 Antibody (C-04) [NB500-306] - Analysis of cytokeratin 18 in HeLa cells with anti-cytokeratin 18 (C-04) biotin.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-C-04-Western-Blot-NB500-306-img0005.jpg "Western Blot: Cytokeratin 18 Antibody (C-04) [NB500-306] - Analysis of cytokeratin 18 in HeLa cells with anti-cytokeratin 18 (C-04) biotin.")
| Reactivity | Hu, Mu, MaSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, IP, CyTOF-ready |
| Clone | C-04 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Cytoskeleton preparation of epidermal carcinoma cell line A431. |
| Marker | Epithelial Marker |
| Specificity | The antibody C-04 reacts with Cytokeratin 18 (45 kDa), a member of intermediate filaments subfamily represented in epithelial tissues. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT18 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Predicted molecular weight: 45 kDa. Western Blot - Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (10% separating gel) ELISA - The antibody C-04 has been tested as the capture antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with antibody DA-7 and with the antibody DC-10. This antibody is CyTOF ready. |
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| Theoretical MW | 45 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS (pH 7.4) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 18 Antibody (NB500-306)Find related products by research area.
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Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen... Read full blog post. |
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| Gene Symbol | KRT18 |
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04) [NB500-306] - Immunohistochemistry staining of human kidney (paraffin-embedded sections) with anti-cytokeratin 18 (C-04). Image using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-C-04-Immunohistochemistry-Paraffin-NB500-306-img0008.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04) [NB500-306] - Immunohistochemistry staining of human kidney (paraffin-embedded sections) with anti-cytokeratin 18 (C-04). Image using the Biotin format of this antibody.")
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