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Cytokeratin 15 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody [NBP1-85602] - Analysis in human skin and kidney tissues. Corresponding KRT15 RNA-seq data are presented for the same tissues.
Orthogonal Strategies: Western Blot: Cytokeratin 15 Antibody [NBP1-85602] - Analysis in human cell line A-431 and human cell line HEK 293.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 15 Antibody [NBP1-85602] - Staining of human cell line HaCaT shows localization to nucleoplasm & intermediate filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody [NBP1-85602] - Staining of human skin shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody [NBP1-85602] - Staining of human fallopian tube shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody [NBP1-85602] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody [NBP1-85602] - Staining of human kidney shows no positivity in cells in tubules as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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Cytokeratin 15 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TTTFLQTSSSTFGGGSTRGGSLLAGGGGFGGGSLSGGGGSRSISASSARFVSSGSGGGYG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KRT15
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Cytokeratin 15 Protein (NBP1-85602PEP)
Publications
Read Publication using NBP1-85602.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (83%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Cytokeratin 15 Antibody

  • CK15
  • CK-15
  • cytokeratin 15
  • Cytokeratin-15
  • K15keratin-15, basic
  • K1CO
  • keratin 15
  • keratin, type I cytoskeletal 15
  • Keratin-15
  • keratin-15, beta
  • KRTB
  • type I cytoskeletal 15

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC

Publications for Cytokeratin 15 Antibody (NBP1-85602)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cytokeratin 15 Antibody (NBP1-85602) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol KRT15