![Western Blot: Cytokeratin 15 Antibody (ST04-85) [NBP2-67525] - Analysis of Cytokeratin 15 on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Cytokeratin-15-Antibody-ST04-85-Western-Blot-NBP2-67525-img0003.jpg "Western Blot: Cytokeratin 15 Antibody (ST04-85) [NBP2-67525] - Analysis of Cytokeratin 15 on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | ST04-85 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human Cytokeratin 15 aa 410-455 / 456. (SwissProt: P19012 Human; SwissProt: Q61414 Mouse; SwissProt: Q6IFV3 Rat) |
| Localization | Intermediate filament, cytosol, extracellular exosome, nucleus. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | KRT15 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | Use in ICC/IF reported in scientific literature (PMID:35664254) . |
|
| Publications |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Choline Kinase beta [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Choline Kinase beta [Unconjugated]")
| Publications using NBP2-67525 | Applications | Species |
|---|---|---|
| Eriksen A, Moller R, Markovoz B et al. Protocols for SARS-CoV-2 infection in primary ocular cells and eye organoids STAR Protocols 2022-04-01 [PMID: 35664254] (ICC/IF, Human) | ICC/IF | Human |
| Wu J, Crowe DL PARP5B is required for nonhomologous end joining during tumorigenesis in vivo Molecular carcinogenesis 2021-10-28 [PMID: 34710250] | ||
| Eriksen AZ, Moller R, Makovoz B et al. SARS-CoV-2 infects human adult donor eyes and hESC-derived ocular epithelium Cell stem cell 2021-05-17 [PMID: 34022129] (IF/IHC, Human) Details: Immunohistochemistry was performed using the Cytokeratin 15 antibody on post-mortem human ocular surface tissue. These immunofluorescent images a visual of the limbal region in SARS-CoV-2-positive cases. |
IF/IHC | Human |
| Odibeli C Nicotinamide Prevents Cytokine-Induced Epithelial-to-Mesenchymal Transition in an in-vitro Model of Corneal Scar Pathophysiology Thesis 2022-01-01 |
Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 15 Antibody (NBP2-67525)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | KRT15 |
![Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (ST04-85) [NBP2-67525] - Analysis of paraffin-embedded human skin tissue with Rabbit anti-Cytokeratin 15 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-15-Antibody-ST04-85-Immunohistochemistry-Paraffin-NBP2-67525-img0007.jpg "Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (ST04-85) [NBP2-67525] - Analysis of paraffin-embedded human skin tissue with Rabbit anti-Cytokeratin 15 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (ST04-85) [NBP2-67525] - Analysis of paraffin-embedded mouse skin tissue using anti-Cytokeratin 15 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-15-Antibody-ST04-85-Immunohistochemistry-Paraffin-NBP2-67525-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (ST04-85) [NBP2-67525] - Analysis of paraffin-embedded mouse skin tissue using anti-Cytokeratin 15 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (ST04-85) [NBP2-67525] - Analysis of paraffin-embedded mouse small intestine tissue using anti-Cytokeratin 15 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-15-Antibody-ST04-85-Immunohistochemistry-Paraffin-NBP2-67525-img0002.jpg "Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (ST04-85) [NBP2-67525] - Analysis of paraffin-embedded mouse small intestine tissue using anti-Cytokeratin 15 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (ST04-85) [NBP2-67525] - Human skin tissue labeling Cytokeratin 15. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 15 ( red) at 1/500 dilution at +4c overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 594, was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-15-Antibody-ST04-85-Immunohistochemistry-Paraffin-NBP2-67525-img0004.jpg "Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (ST04-85) [NBP2-67525] - Human skin tissue labeling Cytokeratin 15. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 15 ( red) at 1/500 dilution at +4c overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 594, was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (ST04-85) [NBP2-67525] - Rat skin tissue labeling Cytokeratin 15. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 15 ( red) at 1/500 dilution at +4? overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 594,) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-15-Antibody-ST04-85-Immunohistochemistry-Paraffin-NBP2-67525-img0005.jpg "Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (ST04-85) [NBP2-67525] - Rat skin tissue labeling Cytokeratin 15. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 15 ( red) at 1/500 dilution at +4? overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 594,) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (ST04-85) [NBP2-67525] - Human skin tissue labeling Cytokeratin 15 and Vimentin. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 15 (red) at 1/400 dilution and Vimentin (EM0401, green) at 1/400 dilution at +4c overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 594, and Goat Anti-Mouse IgG H&L (iFluor™ 488,) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-15-Antibody-ST04-85-Immunohistochemistry-Paraffin-NBP2-67525-img0006.jpg "Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (ST04-85) [NBP2-67525] - Human skin tissue labeling Cytokeratin 15 and Vimentin. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 15 (red) at 1/400 dilution and Vimentin (EM0401, green) at 1/400 dilution at +4c overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 594, and Goat Anti-Mouse IgG H&L (iFluor™ 488,) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunocytochemistry Nestin Antibody (196908) [Unconjugated]](https://images.novusbio.com/images2/Nestin_MAB1259_Immunocytochemistry_6561.jpg)
![Intracellular Staining by Flow Cytometry Nestin Antibody (196908) [Unconjugated]](https://images.novusbio.com/images/antibody/mab1259_human-nestin-mab-clone-196908-intracellular-staining-by-flow-cytometry-24120239959.jpg)
![Western Blot Nestin Antibody (196908) [Unconjugated]](https://images.novusbio.com/images/mab1259_human-nestin-mab-clone-196908-41202410481129.jpg)
![Immunohistochemistry CD34 Antibody [Unconjugated]](https://images.novusbio.com/images2/CD34_AF4117_Immunohistochemistry_6824.jpg)
![Immunohistochemistry p63/TP73L Antibody [Unconjugated]](https://images.novusbio.com/images2/p63_AF1916_Immunohistochemistry_16919.jpg)
![Immunocytochemistry p63/TP73L Antibody [Unconjugated]](https://images.novusbio.com/images2/p63_AF1916_Immunocytochemistry__Immunofluorescence_16973.jpg)
![Immunocytochemistry/ Immunofluorescence p63/TP73L Antibody [Unconjugated]](https://images.novusbio.com/images/af1916_human-p63-tp73l-affinity-purified-polyclonal-ab-41202412401237.jpg)
![Immunocytochemistry CKAP4/p63 Antibody [Unconjugated]](https://images.novusbio.com/images2/CKAP4_AF7355_Immunocytochemistry__Immunofluorescence_17000.jpg)
![Western Blot CKAP4/p63 Antibody [Unconjugated]](https://images.novusbio.com/images2/CKAP4_AF7355_Western_Blot_11997.jpg)
![Immunocytochemistry CKAP4/p63 Antibody [Unconjugated]](https://images.novusbio.com/images2/CKAP4_AF7355_Immunocytochemistry_12002.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")