![Western Blot: Cytochrome P450 3A4 Antibody [NB600-1396] - CYP3A4 (A) and HMG-CoA (B) levels from liver samples from chronically administered animals (14 days) were quantified by ELISA and western blots. Beta-actin served as a reference signal for CYP3A4 (C) and HMG-CoA Reductase (D). Error bars signify standard error of the mean.Citation: Buchanan B, Meng Q, Poulin M-M, Zuccolo J, Azike CG, Gabriele J, et al. (2018) Comparative pharmacokinetics and safety assessment of transdermal berberine and dihydroberberine. PLoS ONE 13(3): e0194979. //doi.org/10.1371/journal.pone.0194979](http://images.novusbio.com/fullsize/Cytochrome-P450-3A4-Antibody-Western-Blot-NB600-1396-img0009.jpg "Western Blot: Cytochrome P450 3A4 Antibody [NB600-1396] - CYP3A4 (A) and HMG-CoA (B) levels from liver samples from chronically administered animals (14 days) were quantified by ELISA and western blots. Beta-actin served as a reference signal for CYP3A4 (C) and HMG-CoA Reductase (D). Error bars signify standard error of the mean.Citation: Buchanan B, Meng Q, Poulin M-M, Zuccolo J, Azike CG, Gabriele J, et al. (2018) Comparative pharmacokinetics and safety assessment of transdermal berberine and dihydroberberine. PLoS ONE 13(3): e0194979. //doi.org/10.1371/journal.pone.0194979")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Purified, His-tagged, full length human P450 3A4 fusion protein. |
| Specificity | Detects Cytochrome P450 (P450) 3A4 from human and rat tissues and cells. This detects, to a lesser extent, other P450 isoforms. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CYP3A4 |
| Purity | Ammonium sulfate precipitation |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Detects an approx. 46 kDa protein representing P450 3A4 from rat liver extracts. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS with 1 mg/ml BSA |
| Preservative | 0.05% Sodium Azide |
| Purity | Ammonium sulfate precipitation |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: Cytochrome P450 3A4 Antibody [NB600-1396] - Analysis of rat liver extract.](http://images.novusbio.com/fullsize/Cytochrome-P450-3A4-Antibody-Western-Blot-NB600-1396-img0005.jpg "Western Blot: Cytochrome P450 3A4 Antibody [NB600-1396] - Analysis of rat liver extract.")
![Western Blot: Cytochrome P450 3A4 Antibody [NB600-1396] - Analysis of from rat liver extract.](http://images.novusbio.com/fullsize/Cytochrome-P450-3A4-Antibody-Western-Blot-NB600-1396-img0006.jpg "Western Blot: Cytochrome P450 3A4 Antibody [NB600-1396] - Analysis of from rat liver extract.")
![Western Blot: Cytochrome P450 3A4 Antibody [NB600-1396] - Analysis was performed on whole celle extracts (30ug lysate) of Mouse Liver (Lane 1) and Rat Liver (Lane 2).](http://images.novusbio.com/fullsize/Cytochrome-P450-3A4-Antibody-Western-Blot-NB600-1396-img0008.jpg "Western Blot: Cytochrome P450 3A4 Antibody [NB600-1396] - Analysis was performed on whole celle extracts (30ug lysate) of Mouse Liver (Lane 1) and Rat Liver (Lane 2).")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")