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Cytochrome P450 2E1 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85367] - Analysis in human liver and endometrium tissues. Corresponding CYP2E1 RNA-seq data are presented for the same ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85367] - Staining of human endometrium, liver, liver cancer and tonsil using Anti-CYP2E1 antibody NBP1-85367 (A) shows ...read more
Western Blot: Cytochrome P450 2E1 Antibody [NBP1-85367] - Western blot analysis in mouse liver tissue and rat liver tissue.
Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85367] - Staining of human endometrium shows no positivity in glandular or stromal cells as expected.
Western Blot: Cytochrome P450 2E1 Antibody [NBP1-85367] - Analysis in human liver tissue.
Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85367] - Staining of human liver cancer shows moderate cytoplasmic positivity in tumor cells.
Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85367] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85367] - Staining of human tonsil shows no positivity in germinal center cells as expected.
Western Blot: Rabbit Polyclonal Cytochrome P450 2E1 Antibody [NBP1-85367] - Western Blot of the liver homogenates from control and alcohol-fed mice. Image from a verified customer review.
Western Blot: Rabbit Polyclonal Cytochrome P450 2E1 Antibody [NBP1-85367] - Cytochrome P450 2E1 western blot of the homogenates from control and alcohol-fed rats. Image from a verified customer review.
Western Blot: Rabbit Polyclonal Cytochrome P450 2E1 Antibody [NBP1-85367] - Cytochrome P450 2E1 western blot of the lysate of VL-17A cells (Hep G2 cells that stably express both Cytochrome P450 2E1 and ADH): control and ...read more
Immunocytochemistry/ Immunofluorescence: Rabbit Polyclonal Cytochrome P450 2E1 Antibody [NBP1-85367] - VA-13 cells (mouse ADH1-transfected HepG2 cells) stained with Cytochrome P450 2E1 Antibody. Image from a verified ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, V-ViSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Cytochrome P450 2E1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EKLHEEIDRVIGPSRIPAIKDRQEMPYMDAVVHEIQRFITLVPSNLPHEATRDTIFRGYLIPKGTVVVPTLDSVLYDNQEFPDPEKFKPEHFLNENGKFKYSDYFKPFSTGKRVCAG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CYP2E1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence Validated from a verified customer review.
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Cytochrome P450 2E1 Protein (NBP1-85367PEP)
Reviewed Applications
Read 5 Reviews rated 5
using
NBP1-85367 in the following applications:

Publications
Read Publications using
NBP1-85367 in the following applications:

Reactivity Notes

Virus reactivity reported in scientific literature (PMID: 30185779).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Cytochrome P450 2E1 Antibody

  • 4-nitrophenol 2-hydroxylase
  • CPE1
  • CYP2Ecytochrome P450 2E1
  • CYPIIE1
  • cytochrome P450, family 2, subfamily E, polypeptide 1
  • cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1
  • Cytochrome P450-J
  • EC 1.14.13.-
  • EC 1.14.13.n7
  • EC 1.14.14.1
  • flavoprotein-linked monooxygenase
  • microsomal monooxygenase
  • P450C2E
  • P450-J
  • xenobiotic monooxygenase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, V-Vi
Applications: WB, ICC/IF, IHC

Publications for Cytochrome P450 2E1 Antibody (NBP1-85367)(4)

Reviews for Cytochrome P450 2E1 Antibody (NBP1-85367) (5) 55

Average Rating: 5
(Based on 5 reviews)
We have 5 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using NBP1-85367:
Filter by Applications
WB
(3)
Immunocytochemistry/Immunofluorescence
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(2)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Armen Petrosyan
WB Human 11/10/2024
View

Summary

ApplicationWestern Blot
Sample TestedHepG2 CELLS
SpeciesHuman
Lot000045420
  5
reviewed by:
Armen Petrosyan
WB Rat 11/09/2024
View

Summary

ApplicationWestern Blot
Sample TestedRat hepatocytes
SpeciesRat
Lot000045420
  5
reviewed by:
Armen Petrosyan
Immunocytochemistry/Immunofluorescence Human 11/06/2024
View

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedHepG2 hepatoma cells
SpeciesHuman
Lot000045420

Comments

Comments***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***
  5
reviewed by:
Armen Petrosyan
WB Mouse 11/06/2024
View

Summary

ApplicationWestern Blot
Sample TestedLiver homogenates sample
SpeciesMouse
Lot000045420
Immunohistochemistry-Paraffin Cytochrome P450 2E1 NBP1-85367
Enlarge
5
reviewed by:
Marta Melis
IHC-P Mouse 12/21/2019
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedmouse liver FFPE section,mouse liver paraffin section
SpeciesMouse
LotB115936

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cytochrome P450 2E1 Antibody (NBP1-85367) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
5
4
0
3
0
2
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1
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Armen Petrosyan
11/10/2024
Application: WB
Species: Human

Armen Petrosyan
11/09/2024
Application: WB
Species: Rat

Armen Petrosyan
11/06/2024
Application: Immunocytochemistry/Immunofluorescence
Species: Human

Bioinformatics

Gene Symbol CYP2E1