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GSTT1 Antibody

Images

 
Immunohistochemistry-Paraffin: GSTT1 Antibody [NBP1-32733] - Human hepatoma. GSTT1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: GSTT1 Antibody [NBP1-32733] - A. 50 ug rat liver lysate/extract 12 % SDS-PAGE GSTT1 antibody dilution: 1:500
Western Blot: GSTT1 Antibody [NBP1-32733] - GSTT1 antibody detects GSTT1 protein by Western blot analysis. Whole cell extracts (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with GSTT1 antibody ...read more
Western Blot: GSTT1 Antibody [NBP1-32733] - GSTT1 antibody detects GSTT1 protein by Western blot analysis. Mouse tissue extracts (50 ug) was separated by 12 % SDS-PAGE, and the membrane was blotted with GSTT1 antibody ...read more
Immunoprecipitation: GSTT1 Antibody [NBP1-32733] - Immunoprecipitation of GSTT1 protein from HepG2 whole cell extracts using 5 ug of GSTT1 antibody. Western blot analysis was performed using GSTT1 antibody. EasyBlot ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, RMSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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GSTT1 Antibody Summary

Immunogen
Full length human GSTT1 Recombinant protein.
Localization
Cytoplasm
Predicted Species
Rhesus Macaque (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GSTT1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for GSTT1 Antibody

  • EC 2.5.1.18
  • glutathione S-transferase theta 1
  • glutathione S-transferase theta-1
  • Glutathione transferase T1-1
  • GST class-theta-1

Background

Glutathione S-transferase (GST) theta 1 (GSTT1) is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1 and GSTT2. The GSTT1 and GSTT2 share 55% amino acid sequence identity and both of them were claimed to have an important role in human carcinogenesis. The GSTT1 gene is located approximately 50kb away from the GSTT2 gene. The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GSTT1