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Cytochrome P450 2B6 Antibody (OTI3D5) - Azide and BSA Free

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Western Blot: Cytochrome P450 2B6 Antibody (OTI3D5) - Azide and BSA Free [NBP2-70526] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 2B6 (Right lane) cDNA for 48 ...read more
Immunocytochemistry/ Immunofluorescence: Cytochrome P450 2B6 Antibody (OTI3D5) - Azide and BSA Free [NBP2-70526] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytochrome P450 2B6.
Immunohistochemistry: Cytochrome P450 2B6 Antibody (OTI3D5) - Azide and BSA Free [NBP2-70526] - Staining of paraffin-embedded Human Kidney tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.
Flow Cytometry: Cytochrome P450 2B6 Antibody (OTI3D5) - Azide and BSA Free [NBP2-70526] - Staining of paraffin-embedded Human liver tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.
Western Blot: Cytochrome P450 2B6 Antibody (OTI3D5) - Azide and BSA Free [NBP2-70526] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Cytochrome P450 2B6 monoclonal antibody (HepG2: human; HeLa: ...read more
Immunohistochemistry: Cytochrome P450 2B6 Antibody (OTI3D5) - Azide and BSA Free [NBP2-70526] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Cytochrome P450 2B6 mouse monoclonal ...read more
Immunohistochemistry: Cytochrome P450 2B6 Antibody (OTI3D5) - Azide and BSA Free [NBP2-70526] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.
Immunohistochemistry: Cytochrome P450 2B6 Antibody (OTI3D5) - Azide and BSA Free [NBP2-70526] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.
Immunohistochemistry: Cytochrome P450 2B6 Antibody (OTI3D5) - Azide and BSA Free [NBP2-70526] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.
Immunohistochemistry: Cytochrome P450 2B6 Antibody (OTI3D5) - Azide and BSA Free [NBP2-70526] - Staining of paraffin-embedded Human colon tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.
Flow Cytometry: Cytochrome P450 2B6 Antibody (OTI3D5) - Azide and BSA Free [NBP2-70526] - Analysis of Jurkat cells, using anti-Cytochrome P450 2B6 antibody, (Red), compared to a nonspecific negative control antibody ...read more
Flow Cytometry: Cytochrome P450 2B6 Antibody (OTI3D5) - Azide and BSA Free [NBP2-70526] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI3D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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Cytochrome P450 2B6 Antibody (OTI3D5) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human CYP2B6(NP_000758) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CYP2B6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
Theoretical MW
56.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Cytochrome P450 2B6 Antibody (OTI3D5) - Azide and BSA Free

  • CPB6
  • CYP2B
  • CYP2B6
  • CYP2B7
  • CYP2B7P
  • CYPIIB6
  • CYPIIB6cytochrome P450, subfamily IIB (phenobarbital-inducible)
  • Cytochrome P450 2B6
  • Cytochrome P450 IIB1
  • cytochrome P450, family 2, subfamily B
  • cytochrome P450, family 2, subfamily B, polypeptide 6
  • cytochrome P450, subfamily IIB (phenobarbital-inducible), polypeptide 6
  • EC 1.14.13.-
  • EC 1.14.14.1
  • IIB1
  • P450

Background

The Cytochrome P450 2B6 gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This prot

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CYP2B6