Home » Cystatin C » Cystatin C Antibodies » Cystatin C Antibody [Unconjugated]
Novus Biologicals products are now on bio-techne.com

Cystatin C Antibody [Unconjugated]

 (4 Publications)
 
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, human liver tissue, and human serum. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Cystatin C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1196) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Cystatin C at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, human liver tissue, and human serum. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Cystatin C Antigen Affinity-purified ...read more
Western blot shows lysates of human placenta tissue and PC-3 human prostate cancer cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human Cystatin C Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Cystatin C was detected in immersion fixed paraffin-embedded sections of human breast using 5 µg/mL Goat Anti-Human Cystatin C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1196) overnight at 4 °C. Tissue ...read more
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Cystatin C at approximately 20 kDa (as indicated) using 10 µg/mL of ...read more
Cystatin E/M and C secretions from various cell lines: Common laboratory non-melanoma and established laboratory melanoma cell lines (top), and primary and metastatic melanoma cell lines established from patients, as ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, IP, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF1196
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF1196-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Cystatin C Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cystatin C
Ser27-Ala146
Accession # P01034
Specificity
Detects human Cystatin C in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse Cystatin C is observed and less than 5% cross-reactivity with recombinant human (rh) Fetuin A, rhFetuin B, rhCystatin A, rhCystatin B, rhCystatin S, and rhCystatin E/M is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CST3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Neutralization 12-112 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.25-2 ug/mL
Publications
Read Publications using
AF1196 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cystatin C Antibody [Unconjugated]

  • ARMD11
  • bA218C14.4 (cystatin C)
  • CST3
  • cystatin 3
  • cystatin C (amyloid angiopathy and cerebral hemorrhage)
  • Cystatin C
  • cystatin-3
  • cystatin-C
  • Gamma-trace
  • MGC117328
  • Neuroendocrine basic polypeptide
  • Post-gamma-globulin

Background

Cystatin C is a member of family 2 of the Cystatin superfamily (1). It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L, and S (2, 3). It is ubiquitous in human tissues and body fluids. A point mutation in the gene coding for the 120 amino acid mature Cystatin C causes a hereditary form of amyloid angiopathy in which the protein variant (Leu68 to Gln) is deposited in the cerebral arteries, leading to fatal cerebral hemorrhage (4). Cystatin C may have additional clinical applications. For example, it is a good marker for glomerular filtration rate (5).

  1. Reed, C.H. (2000) British J. Biomed. Sci. 57:323.
  2. Janowski, R. et al. (2001) Nat. Struct. Biol. 8:316.
  3. Abrahamson, M. (1994) Methods Enzymol. 244:685.
  4. Abrahamson, M. et al. (1992) Hum. Genet. 89:377.
  5. Laterza, O.F. et al. (2002) Clin. Chem. 48:699.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

RAPGEF5 Antibody
NBP2-57362
Western Blot RAPGEF5 Antibody
Immunocytochemistry/ Immunofluorescence RAPGEF5 Antibody
Species: Hu
Applications: ICC/IF, WB
Lipocalin-2/NGAL
[Biotin]
DY1857
N/A Lipocalin-2/NGAL [Biotin]
(135 Publications)
Species: Mu
Applications: ELISA
Albumin Antibody
(188835) [Unconjugate ...
MAB1455
Simple Western Albumin Antibody (188835) [Unconjugated] - Serum
Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum
Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum
(3 Reviews)
(54 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
C-Reactive
Protein/CRP [Biotin]
DY1707
N/A C-Reactive Protein/CRP [Biotin]
(70 Publications)
Species: Hu
Applications: ELISA
Cathepsin B
Antibody [Unconjugate
AF965
Immunohistochemistry Cathepsin B Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence Cathepsin B Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence Cathepsin B Antibody [Unconjugated]
(1 Review)
(51 Publications)
Species: Mu
Applications: IHC, Neut, WB
Cathepsin L
Antibody [Unconjugate
AF952
Western Blot Cathepsin L Antibody [Unconjugated]
Immunohistochemistry Cathepsin L Antibody [Unconjugated]
Western Blot Cathepsin L Antibody [Unconjugated]
(27 Publications)
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, IP, WB
Cystatin E/M/CST6
Antibody [Unconjugate ...
AF1286
Western Blot Cystatin E/M/CST6 Antibody [Unconjugated]
Simple Western Cystatin E/M/CST6 Antibody [Unconjugated]
Western Blot Cystatin E/M/CST6 Antibody [Unconjugated]
(1 Review)
(6 Publications)
Species: Hu
Applications: IHC, IP, Simple Western, WB
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(670 Publications)
Species: Mu
Applications: ELISA
GM-CSF
[Unconjugated]
7954-GM/CF
Bioactivity GM-CSF [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
LAMC2 Antibody
(CL2980)
NBP2-42388
Western Blot LAMC2 Antibody (CL2980)
Immunohistochemistry-Paraffin LAMC2 Antibody (CL2980)
Immunohistochemistry-Paraffin LAMC2 Antibody (CL2980)
(4 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
CD55/DAF Antibody
[Unconjugated]
AF2009
Immunohistochemistry CD55/DAF Antibody [Unconjugated]
Immunocytochemistry CD55/DAF Antibody [Unconjugated]
(8 Publications)
Species: Hu
Applications: ICC, IHC
Calretinin Antibody
NBP1-88220
Immunocytochemistry/ Immunofluorescence Calretinin Antibody
Western Blot Calretinin Antibody
Immunohistochemistry-Paraffin Calretinin Antibody
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
CD21 Antibody
(SC0681)
NBP2-67605
Immunocytochemistry/ Immunofluorescence CD21 Antibody (SC0681)
Immunocytochemistry/ Immunofluorescence CD21 Antibody (SC0681)
Immunohistochemistry-Paraffin CD21 Antibody (SC0681)
(1 Publication)
Species: Hu, Pm, Mu
Applications: ICC/IF, IHC, IHC-P, IP, ISH, WB
DHRS4 Antibody
NBP2-98675
Immunohistochemistry-Paraffin DHRS4 Antibody
Immunohistochemistry-Paraffin DHRS4 Antibody
Western Blot DHRS4 Antibody
Species: Hu
Applications: IHC-P, IP, WB
CRIPTO Antibody
[Unconjugated]
AF1538
Immunocytochemistry CRIPTO Antibody [Unconjugated]
(4 Publications)
Species: Mu
Applications: Block, ICC, WB
IL-18/IL-1F4 [HRP]
7625
IL-18/IL-1F4 [HRP]
(54 Publications)
Species: Mu
Applications: ELISA
TIM-1/KIM-1/HAVCR
Antibody [Unconjugate ...
AF1817
Western Blot TIM-1/KIM-1/HAVCR Antibody [Unconjugated]
Western Blot TIM-1/KIM-1/HAVCR Antibody [Unconjugated]
(105 Publications)
Species: Mu
Applications: WB
beta Amyloid
Antibody (MOAB-2) - B ...
NBP2-13075
Immunohistochemistry-Frozen beta Amyloid Antibody (MOAB-2) - BSA Free
Immunohistochemistry beta Amyloid Antibody (MOAB-2) - BSA Free
Immunocytochemistry/ Immunofluorescence beta Amyloid Antibody (MOAB-2) - BSA Free
(2 Reviews)
(70 Publications)
Species: Ba, Hu, Pm, Mu, Rt
Applications: DB, ELISA, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, WB
CSRP1 Antibody
AF5739
Western Blot CSRP1 Antibody
Species: Hu, Mu, Rt
Applications: WB
Cystatin C
Antibody [Unconjugate
AF1196
Cystatin C Antibody [Unconjugated]
Species: Hu
Applications: WB, Simple Western, IHC, IP, B/N

Publications for Cystatin C Antibody (AF1196)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Array Development, ELISA Development, Western Blot.


Filter By Application
Array Development
(1)
ELISA Development
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(3)
All Species

Reviews for Cystatin C Antibody (AF1196) (0)

There are no reviews for Cystatin C Antibody (AF1196). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Cystatin C Antibody (AF1196) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Cystatin C Products

Blogs on Cystatin C

There are no specific blogs for Cystatin C, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Cystatin C Antibody [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol CST3
Uniprot