![Western Blot: CYFIP1 Antibody [NBP2-16060] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CYFIP1 antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CYFIP1-Antibody-Western-Blot-NBP2-16060-img0009.jpg "Western Blot: CYFIP1 Antibody [NBP2-16060] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CYFIP1 antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Bv, ZeSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human CYFIP1. The exact sequence is proprietary. |
| Localization | Cytoplasm; perinuclear region; Cell projection; lamellipodium; ruffle; Cell junction; synapse; synaptosome |
| Predicted Species | Bovine (100%), Zebrafish (93%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CYFIP1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 145 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
![Neutralization CXCL8/IL-8 Antibody (6217) [Unconjugated]](https://images.novusbio.com/images2/CXCL8_MAB208_Block_Neutralize_9064.jpg)
![Immunocytochemistry CXCL8/IL-8 Antibody (6217) [Unconjugated]](https://images.novusbio.com/images2/CXCL8_MAB208_Immunocytochemistry_9339.jpg)
![Western Blot CXCL8/IL-8 Antibody (6217) [Unconjugated]](https://images.novusbio.com/images2/mab208_human-il-8-cxcl8-mab-clone-6217-western-blot-1882021143847.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: CYFIP1 Antibody [NBP2-16060] - DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CYFIP1 stained by CYFIP1 antibody [N1N2], N-term diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/CYFIP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16060-img0007.jpg "Immunocytochemistry/Immunofluorescence: CYFIP1 Antibody [NBP2-16060] - DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CYFIP1 stained by CYFIP1 antibody [N1N2], N-term diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: CYFIP1 Antibody [NBP2-16060] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CYFIP1-Antibody-Immunohistochemistry-Paraffin-NBP2-16060-img0003.jpg "Immunohistochemistry-Paraffin: CYFIP1 Antibody [NBP2-16060] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.")
![Western Blot: CYFIP1 Antibody [NBP2-16060] - Sample (30 ug of whole cell lysate) A: HeLa 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CYFIP1-Antibody-Western-Blot-NBP2-16060-img0002.jpg "Western Blot: CYFIP1 Antibody [NBP2-16060] - Sample (30 ug of whole cell lysate) A: HeLa 5% SDS PAGE gel, diluted at 1:1000.")
![Immunoprecipitation: CYFIP1 Antibody [NBP2-16060] - Immunoprecipitation of CYFIP1 protein from HeLa whole cell extracts using 5 ug of CYFIP1 antibody [N1N2], N-term. Western blot analysis was performed using CYFIP1 antibody [N1N2], N-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/CYFIP1-Antibody-Immunoprecipitation-NBP2-16060-img0006.jpg "Immunoprecipitation: CYFIP1 Antibody [NBP2-16060] - Immunoprecipitation of CYFIP1 protein from HeLa whole cell extracts using 5 ug of CYFIP1 antibody [N1N2], N-term. Western blot analysis was performed using CYFIP1 antibody [N1N2], N-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.")
![Western Blot Angiopoietin-like Protein 2/ANGPTL2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Angiopoietinlike_Protein_2_AF1444_Western_Blot_19840.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")