![Western Blot: CYBB/NOX2 Antibody (SN07-16) [NBP2-67680] Analysis of NOX2/gp91phox on different lysates with Rabbit anti-NOX2/gp91phox antibody at 1/2,000 dilution.Lane 1: HepG2 cell lysate (15 ug/Lane)Lane 2: MCF7 cell lysate (15 ug/Lane)Lane 3: Mouse brain tissue lysate (20 ug/Lane)Lane 4: Mouse spleen tissue lysate (20 ug/Lane)Lane 5: Rat brain tissue lysate (20 ug/Lane)Lane 6: Rat spleen tissue lysate (20 ug/Lane)Predicted band size: 65 kDaObserved band size: 55 kDaExposure time: 3 minutes;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67680_rabbit-monoclonal-cybb-nox2-antibody-sn07-16-western-blot-197202491539..jpg "Western Blot: CYBB/NOX2 Antibody (SN07-16) [NBP2-67680] Analysis of NOX2/gp91phox on different lysates with Rabbit anti-NOX2/gp91phox antibody at 1/2,000 dilution.Lane 1: HepG2 cell lysate (15 ug/Lane)Lane 2: MCF7 cell lysate (15 ug/Lane)Lane 3: Mouse brain tissue lysate (20 ug/Lane)Lane 4: Mouse spleen tissue lysate (20 ug/Lane)Lane 5: Rat brain tissue lysate (20 ug/Lane)Lane 6: Rat spleen tissue lysate (20 ug/Lane)Predicted band size: 65 kDaObserved band size: 55 kDaExposure time: 3 minutes;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB |
| Clone | SN07-16 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human CYBB/NOX2 aa 131-180 / 570. (SwissProt: P04839 Human; SwissProt: Q61093 Mouse; Entrez Gene:66021 Rat) |
| Localization | Cell membrane. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CYBB |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for CYBB/NOX2 Antibody (NBP2-67680)Find related products by research area.
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| Gene Symbol | CYBB |
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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