Reactivity | PoSpecies Glossary |
Applications | PAGE, Bioactivity |
Format | Carrier-Free |
Concentration | LYOPH |
Description | A single non-glycosylated polypeptide chain containing 78 amino acids corresponding to CXCL8/IL-8 Source: E. coli Uniprot ID: P26894 Amino Acid Sequence: ARVSAELRCQ CINTHSTPFH PKFIKELRVI ESGPHCENSE IIVKLVNGKE VCLDPKEKWV QKVVQIFLKR TEKQQQQQ |
Details of Functionality | IL8 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration range of 20-200 ng/ml. |
Source | E. coli |
Protein/Peptide Type | Recombinant Protein |
Gene | CXCL8 |
Purity | >95%, by SDS-PAGE and HPLC |
Endotoxin Note | Less than 1 EU/ug of CXCL8/IL-8 as determined by LAL method. |
Dilutions |
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Theoretical MW | 9.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Publications |
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Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Buffer | Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4. |
Preservative | No Preservative |
Concentration | LYOPH |
Purity | >95%, by SDS-PAGE and HPLC |
Reconstitution Instructions | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
Publication using NBP2-35234 | Applications | Species |
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Patel N, Bailey K, Lazenby A, Carlson M Induction of pancreatic neoplasia in the KRAS/TP53 Oncopig: preliminary report bioRxiv 2020-06-02 [PMID: 36579622] |
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Gene Symbol | CXCL8 |