Recombinant Human CXCL8/IL-8 Protein Summary
Description |
A single, non-glycosylated polypeptide chain containing 77 amino acids corresponding to CXCL8/IL-8 Source: E. coli Uniprot ID: P10145 Amino Acid Sequence: AVLPRSAKEL RCQCIKTYSK PFHPKFIKEL RVIESGPHCA NTEIIVKLSD GRELCLDPKE NWVQRVVEKF LKRAENS This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions |
Details of Functionality |
Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human CXCR2 transfected mouse BaF3 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 x 10^5 IU/mg. |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
CXCL8 |
Purity |
>95%, by SDS-PAGE and HPLC |
Endotoxin Note |
Less than 1 EU/ug of CXCL8/IL-8 as determined by LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
8.9 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Buffer |
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
Preservative |
No Preservative |
Concentration |
LYOPH |
Purity |
>95%, by SDS-PAGE and HPLC |
Reconstitution Instructions |
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
Alternate Names for Recombinant Human CXCL8/IL-8 Protein
Background
Interleukin-8 (IL-8) is encoded by the IL8 gene and produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles. There are many receptors capable to bind IL-8, the most affinity to IL-8 are receptors CXCR1, and CXCR2. As a member of the CXC chemokine family, function of IL-8 is the induction of chemotaxis in its target cells, like neutrophil granulocytes, basophils, and T-cells. IL-8 (72 a.a.) has a 5-10 fold higher activity on neutrophil activation, compared to IL-8 (77 a.a.). IL-8 is often associated with inflammation, it has been cited as a proinflammatory mediator in gingivitis and psoriasis
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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