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CXCL8/IL-8 Antibody [Unconjugated]

 (13 Publications)
 (1 Review)
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Recombinant Human IL-8/CXCL8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-8/CXCL8 Polyclonal Antibody (Catalog # AB-208-NA). The ND50 is typically 5-20 µg/mL.
Recombinant Human IL-8/CXCL8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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CXCL8/IL-8 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IL-8/CXCL8
Ser28-Ser99
Accession # P10145
Specificity
Detects human IL-8/CXCL8 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) GRO alpha , rhGRO beta , and rhGRO gamma is observed, and less than 1% cross-reactivity with recombinant feline IL-8/CXCL8, recombinant porcine IL-8/CXCL8, and recombinant canine IL-8/CXCL8 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL8
Purity Statement
Protein A or G purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Neutralization 5-20 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AB-208-NA in the following applications:
Publications
Read Publications using
AB-208-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL8/IL-8 Antibody [Unconjugated]

  • 3-10C
  • AMCF-I
  • C-X-C motif chemokine 8
  • CXCL8
  • CXCL8SCYB8
  • Emoctakin
  • GCP1
  • GCP-1TSG-1
  • IL8
  • IL-8
  • interleukin 8
  • K60
  • LAI
  • LECT
  • LUCT
  • LYNAP
  • MDNCF
  • MDNCFb-ENAP
  • member 8
  • MONAP
  • MONAPGCP1
  • NAF
  • NAP1
  • NAP-1NAP1
  • NCF
  • Neutrophil-activating protein 1
  • Protein 3-10C
  • T cell chemotactic factor
  • T-cell chemotactic factor
  • TCF
  • TSG1

Background

CXCL8 was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce CXCL8 in response to a wide variety of pro-inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. CXCL8 is a member of the alpha (CXC) subfamily of chemokines, which also includes platelet factor 4, GRO, IP-10, etc.

CXCL8 is a potent chemoattractant for neutrophils. In addition, CXCL8 also has a wide range of other pro-inflammatory effects. CXCL8 causes degranulation of neutrophil specific granules and azurophilic granules. CXCL8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, CXCL8 is also chemotactic for basophils, T cells and eosinophils. CXCL8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. CXCL8 was also reported to be angiogenic both in vivo and in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCL8/IL-8 Antibody (AB-208-NA)(13)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: Control, ELISA Development (Capture), Functional Assay, ICC, Neutralization.


Filter By Application
Control
(1)
ELISA Development (Capture)
(1)
Functional Assay
(1)
ICC
(1)
Neutralization
(4)
All Applications
Filter By Species
Human
(9)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AB-208-NA Applications Species
M Patankar, M Li, A Khalatbari, JD Castle, L Hu, C Zhang, A Shaker Inflammatory and Proliferative Pathway Activation in Human Esophageal Myofibroblasts Treated with Acidic Bile Salts International Journal of Molecular Sciences, 2022-09-08;23(18):. 2022-09-08 [PMID: 36142285] (Human) Human
N Sheshadri, DK Poria, S Sharan, Y Hu, C Yan, VN Koparde, K Balamuruga, E Sterneck PERK signaling through C/EBPdelta contributes to ER stress-induced expression of immunomodulatory and tumor promoting chemokines by cancer cells Cell Death & Disease, 2021-11-01;12(11):1038. 2021-11-01 [PMID: 34725321] (Functional Assay, Human) Functional Assay Human
A Angelini, Y Miyabe, D Newsted, BH Kwan, C Miyabe, RL Kelly, MN Jamy, AD Luster, KD Wittrup Directed evolution of broadly crossreactive chemokine-blocking antibodies efficacious in arthritis Nat Commun, 2018-04-13;9(1):1461. 2018-04-13 [PMID: 29654232] (Neutralization, Human) Neutralization Human
Zhijie Lin Salmonella enteritidis Effector AvrA Stabilizes Intestinal Tight Junctions via the JNK Pathway J. Biol. Chem, 2016-11-15;291(52):26837-26849. 2016-11-15 [PMID: 27875307] (ELISA Development (Capture), Human) ELISA Development (Capture) Human
Evidence for chemokine synergy during neutrophil migration in ARDS Thorax, 2016-08-05;0(0):. 2016-08-05 [PMID: 27496101] (Neutralization, Human) Neutralization Human
Manpreet Singh, Anginelle Alabanza, Lorelis E. Gonzalez, Weiwei Wang, W. Brian Reeves, Jong-in Hahm Ultratrace level determination and quantitative analysis of kidney injury biomarkers in patient samples attained by zinc oxide nanorods Nanoscale 2016-02-28 [PMID: 26846189]
Paul Kopesky, Kerstin Tiedemann, Dahlia Alkekhia, Christoph Zechner, Bjorn Millard, Birgit Schoeberl et al. Autocrine signaling is a key regulatory element during osteoclastogenesis Biology Open 2014-08-15 [PMID: 25063197]
Angela Kill, Christoph Tabeling, Reinmar Undeutsch, Anja A Kühl, Jeannine Günther, Mislav Radic et al. Autoantibodies to angiotensin and endothelin receptors in systemic sclerosis induce cellular and systemic events associated with disease pathogenesis Arthritis Research & Therapy 2014-01-28 [PMID: 24472528]
Raquel Rodrigues, Gláucia Paranhos-Baccalà, Guy Vernet, Christophe N. Peyrefitte Crimean-Congo Hemorrhagic Fever Virus-Infected Hepatocytes Induce ER-Stress and Apoptosis Crosstalk PLoS ONE 2012-01-06 [PMID: 22238639]
Sarkar SA, Lee CE, Victorino F, Nguyen TT, Walters JA, Burrack A, Eberlein J, Hildemann SK, Homann D Expression and regulation of chemokines in murine and human type 1 diabetes. Diabetes, 2011-12-30;61(2):436-46. 2011-12-30 [PMID: 22210319] (Control, Human) Control Human
Show All 13 Publications.

Review for CXCL8/IL-8 Antibody (AB-208-NA) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AB-208-NA:
Filter by Applications
ELISA
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
 ELISA Human 01/05/2015
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Summary

ApplicationELISA
Sample TestedSee PMID 19817376
SpeciesHuman

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 Verified Customer
01/05/2015
Application: ELISA
Species: Human
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Bioinformatics

Gene Symbol IL8
Uniprot