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CXCL12/SDF-1 Antibody (79018) [Fluorescein]

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MG-63 human osteosarcoma cell line was stained with Mouse Anti-Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Mono-clonal Antibody (Catalog # IC350F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
MG-63 human osteosarcoma cell line was stained with Mouse Anti-Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Mono-clonal Antibody (Catalog # IC350F, filled histogram) or isotype control antibody (Catalog # IC002F, ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow
Clone
79018
Clonality
Monoclonal
Host
Mouse
Conjugate
Fluorescein

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB350U-100UG
Alexa Fluor 405
FAB350V-100UG
Alexa Fluor 488
IC350G-100
Alexa Fluor 488
FAB350G-100UG
Alexa Fluor 532
FAB350X
Alexa Fluor 594
FAB350T-100UG
Alexa Fluor 647
FAB350R-100UG
Alexa Fluor 700
FAB350N-100UG
Alexa Fluor 750
FAB350S-100UG
Allophycocyanin
IC350A
Biotin
FAB350B
CoraFluor 1
FAB350CL1
DyLight 350
FAB350D
DyLight 405
FAB350E
DyLight 488
FAB350K
DyLight 550
FAB350L
DyLight 594
FAB350M
DyLight 650
FAB350W
DyLight 680
FAB350Y
DyLight 755
FAB350Z
HRP
FAB350H
Janelia Fluor 525
FAB350JF525
Janelia Fluor 549
FAB350I
Janelia Fluor 646
FAB350J
Janelia Fluor 669
FAB350JF669
PerCP (Peridinin-chlorophyll Protein Complex)
IC350C
Phycoerythrin
IC350P
Unconjugated
MAB350-100
mFluor Violet 450 SE
FAB350MFV450
mFluor Violet 500 SE
FAB350MFV500
mFluor Violet 610 SE
FAB350MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB350-100
Carrier Free
IC350C
Carrier Free
IC350P
Carrier Free
IC350A
Carrier Free
MAB350-SP
Carrier Free
MAB350-100
Carrier Free
MAB350-500
Carrier Free
IC350G-100
Carrier Free
FAB350V-100UG
Carrier Free
FAB350U-100UG
Carrier Free
FAB350T-100UG
Carrier Free
FAB350S-100UG
Carrier Free
FAB350R-100UG
Carrier Free
FAB350N-100UG
Carrier Free
FAB350G-100UG
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CXCL12/SDF-1 Antibody (79018) [Fluorescein] Summary

Immunogen
E. coli-derived recombinant human CXCL12/SDF‑1 alpha
Lys22-Lys89
Accession # Q6ICW0
Specificity
Detects human and mouse CXCL12/SDF-1 alpha and CXCL12/SDF-1 beta in ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CXCL12
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry
Publications
Read Publications using
IC350F in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL12/SDF-1 Antibody (79018) [Fluorescein]

  • chemokine (C-X-C motif) ligand 12
  • C-X-C motif chemokine 12
  • CXCL12
  • hIRH
  • hSDF-1
  • Intercrine reduced in hepatomas
  • IRH
  • PBSF
  • PBSFSDF-1a
  • Pre-B cell growth-stimulating factor
  • SCYB12
  • SDF1
  • SDF-1
  • SDF1A
  • SDF1B
  • SDF1SDF-1b
  • stromal cell-derived factor 1
  • TLSF
  • TLSF-a
  • TLSF-b
  • TPAR1

Background

CXCL12, also known as SDF-1, is a CXC chemokine that interacts with CXCR4. SDF-1 alpha and SDF-1 beta are alternatively spliced isoforms of CXCL12. SDF-1 beta has an additional 4 amino acids at the carboxy-terminus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCL12/SDF-1 Antibody (IC350F)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC, IHC-Fr.


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IHC
(1)
IHC-Fr
(1)
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(1)
Mouse
(1)
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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CXCL12
Uniprot