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CXCL10/IP-10/CRG-2 Antibody [Biotin]

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Product Details

Summary
Applications ELISA(Det), Flow
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF-266-NA
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF-266-NA
Carrier Free
AF-266-NA
Carrier Free
AF-266-SP
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CXCL10/IP-10/CRG-2 Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant human CXCL10/IP-10
Specificity
Detects human CXCL10/IP-10/CRG-2 in ELISAs and Western blots. In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant mouse CRG‑2, recombinant human (rh) Lymphotactin, rhGRO alpha , and rhMIG is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CXCL10
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Application Notes
ELISA Capture: Human CXCL10/IP-10/CRG-2 Antibody (Catalog # MAB266)
ELISA Detection: Human CXCL10/IP-10/CRG-2 Biotinylated Antibody (Catalog # BAF266)
Standard: Recombinant Human CXCL10/IP-10/CRG-2 (Catalog # 266-IP)

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

CXCL10 was originally identified as an IFN-gamma -inducible gene in monocytes, fibroblasts, and endothelial cells. It has since been shown that CXCL10 mRNA is also induced by LPS, IL-1 beta , TNF-alpha , IL-12, and viruses. Additional cell types that have been shown to express CXCL10 include activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. CXCL10 is also expressed in psoriatic and lepromatous lesions of skin. The mouse homologue of human CXCL10, CRG-2, has been cloned and shown to share approximately 67% amino acid (aa) sequence identity with human CXCL10. Human CXCL10 cDNA encodes a 98 aa residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted protein. The amino acid sequence of CXCL10 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes. CXCL10 has been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent anti-tumor effect. A chemokine receptor specific for CXCL10 and MIG has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

  1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
  2. Wang, X. et al. (1996) J. Biol. Chem. 271:24286.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CXCL10