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CRX/CORD2 Antibody [Unconjugated]

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Western blot shows lysates of Y-79 human retinoblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CRX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7085) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CRX at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Y-79 human retinoblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CRX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7085) followed by ...read more
CRX was detected in immersion fixed Y-79 human retinoblastoma cell line using Sheep Anti-Human CRX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7085) at 10 µg/mL for 3 hours at room temperature. Cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF7085-SP
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CRX/CORD2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CRX
Ala166-Leu299
Accession # O43186
Specificity
Detects human CRX in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
CRX
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF7085 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CRX/CORD2 Antibody [Unconjugated]

  • cone-rod homeobox
  • CORD2
  • CRDcone-rod homeobox protein
  • CRX
  • LCA7
  • LCA7orthodenticle homeobox 3
  • OTX3

Background

CRX (Cone Rod homeoboX protein) is a 37-39 kDa member of the Otx family of paired-like homeodomain containing proteins. It is expressed in pinealocytes, cones, rods and rod bipolar cells, and promotes the transcription of photoreceptor cell proteins such as rhodopsin, IRBP and beta -phosphodiesterase. Although CRX may act alone, it typically binds noncovalently to both QRX and NRL, forming a transcriptional complex. Human CRX is 299 amino acids (aa) in length. It contains one DNA‑binding homeobox domain (aa 39-98). There is one isoform variant that shows a deletion of aa 146-149. Over aa 166-299, human CRX shares 97% aa identity with mouse CRX.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CRX/CORD2 Antibody (AF7085)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC-Fr, Western Blot.


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IHC-Fr
(1)
Western Blot
(1)
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(1)
Mouse
(1)
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Reviews for CRX/CORD2 Antibody (AF7085) (0)

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CRX
Uniprot