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AIPL1 Antibody (OTI3B4)

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Western Blot: AIPL1 Antibody (3B4) [NBP2-01327] Analysis of extracts (35ug) from 9 different cell lines by using anti-AIPL1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: AIPL1 Antibody (3B4) [NBP2-01327] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY AIPL1.
Immunohistochemistry-Paraffin: AIPL1 Antibody (3B4) [NBP2-01327] - Staining of paraffin-embedded Human pancreas tissue using anti-AIPL1 mouse monoclonal antibody.
Flow Cytometry: AIPL1 Antibody (3B4) [NBP2-01327] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AIPL1 antibody, and then analyzed ...read more
Western Blot: AIPL1 Antibody (3B4) [NBP2-01327] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AIPL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: AIPL1 Antibody (3B4) [NBP2-01327] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-AIPL1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: AIPL1 Antibody (3B4) [NBP2-01327] - Staining of paraffin-embedded Human lymph node tissue using anti-AIPL1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI3B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

AIPL1 Antibody (OTI3B4) Summary

Immunogen
Full length human recombinant protein of human AIPL1(NP_055151) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
AIPL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
43.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AIPL1 Antibody (OTI3B4)

  • AIPL2
  • aryl hydrocarbon receptor interacting protein-like 1
  • aryl hydrocarbon receptor-interacting protein-like 1
  • aryl-hydrocarbon-interacting protein-like 1
  • LCA4

Background

Leber congenital amaurosis (LCA) accounts for at least 5% of all inherited retinal disease and is the most severe inherited retinopathy with the earliest age of onset. Individuals affected with LCA are diagnosed at birth or in the first few months of life with severely impaired vision or blindness, nystagmus and an abnormal or flat electroretinogram. The photoreceptor/pineal -expressed gene, AIPL1, encoding aryl-hydrocarbon interacting protein-like 1, was mapped within the LCA4 candidate region. The protein contains three tetratricopeptide motifs, consistent with nuclear transport or chaperone activity. AIPL1 mutations may cause approximately 20% of recessive LCA. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AIPL1 Antibody (NBP2-01327). (Showing 1 - 1 of 1 FAQ).

  1. For my project I'm interested in an primary antibody that is not currently available on the market. The protein I'm interested in is aryl hydrocarbon receptor interacting protein. I would like to use the antibody for Western Blot and/or Immunoprecipitation, and this antibody has to be specific for a zebra fish protein. I would like to ask for a price quote.
    • We currently have no AIPL1 antibodies validated in Zebra fish. If you are interested in testing in Zebra fish, you would qualify for our Innovators Reward Program. You may also contact us at innovators@novusbio.com should you have any questions about the Innovators Reward Program.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AIPL1
Entrez