Reactivity | MuSpecies Glossary |
Applications | WB |
Clonality | Polyclonal |
Host | Sheep |
Conjugate | Unconjugated |
Concentration | LYOPH |
Immunogen | Chinese hamster ovary cell line CHO-derived recombinant mouse CRISP-4 Leu23-Lys250 Accession # NP_084309 |
Specificity | Detects mouse CRISP-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant mouse CRISP-1, -2, and -3 is observed. |
Source | N/A |
Isotype | IgG |
Clonality | Polyclonal |
Host | Sheep |
Gene | Crisp4 |
Purity Statement | Antigen Affinity-purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Publications |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Preservative | No Preservative |
Concentration | LYOPH |
Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
CRISP‑4 (cysteine‑rich secretory protein 4) is a secreted, 31 kDa member of the CRISP family of proteins. In mouse, its expression is limited to the epididymis, seminal vesicle and vas deferens and it appears to bind to differentiating sperm. Mature mouse CRISP‑4 is 230 amino acids (aa) in length. It contains an SCP (sperm coating protein) region (aa 42‑178) followed by a C‑terminal CRISP domain (aa 196‑250). There appears to be an alternate start site four amino acids upstream of the standard start site. Over aa 22‑550, mouse CRISP‑4 shares 90% aa identity with rat CRISP‑4 and 64% aa identity with human CRISP‑1, the human ortholog to mouse CRISP‑4.
Secondary Antibodies |
Isotype Controls |
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