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CRISP-4 Antibody [Unconjugated]

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Western blot shows lysates of mouse epididymis tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Mouse CRISP-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5017) followed by HRP-conjugated ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price
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CRISP-4 Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse CRISP-4
Leu23-Lys250
Accession # NP_084309
Specificity
Detects mouse CRISP-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant mouse CRISP-1, -2, and -3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
Crisp4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL
Publications
Read Publication using
AF5017 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CRISP-4 Antibody [Unconjugated]

  • CRISP4
  • CRISP-4

Background

CRISP‑4 (cysteine‑rich secretory protein 4) is a secreted, 31 kDa member of the CRISP family of proteins. In mouse, its expression is limited to the epididymis, seminal vesicle and vas deferens and it appears to bind to differentiating sperm. Mature mouse CRISP‑4 is 230 amino acids (aa) in length. It contains an SCP (sperm coating protein) region (aa 42‑178) followed by a C‑terminal CRISP domain (aa 196‑250). There appears to be an alternate start site four amino acids upstream of the standard start site. Over aa 22‑550, mouse CRISP‑4 shares 90% aa identity with rat CRISP‑4 and 64% aa identity with human CRISP‑1, the human ortholog to mouse CRISP‑4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CRISP-4 Antibody (AF5017)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol Crisp4
Uniprot